Anti-ZNF333 Rabbit Polyclonal Antibody for WB, ELISA - Q96JL9

Core Product Specifications and Parameters

Biological Background: ZNF333 Function and Localization

• ZNF333 (Zinc finger protein 333), also known as KIAA1806, is a member of the krueppel C2H2-type zinc-finger protein family, characterized by multiple DNA-binding zinc finger repeats.
• It is predicted to function as a transcriptional regulator, likely involved in modulating gene expression through specific DNA sequence recognition.
• ZNF333 localizes to the nucleus, consistent with its role in transcription.
• The protein contains conserved C2H2 zinc finger motifs that coordinate zinc ions, essential for its DNA-binding activity.
• Alternative splicing generates multiple isoforms of ZNF333, which may exhibit distinct regulatory properties.
• ZNF333 is highly expressed in heart tissue, suggesting a potential role in cardiac gene regulation.
• As a member of the zinc finger transcription factor family, ZNF333 contributes to proteomics-identified reference proteome datasets.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1-303) of human ZNF333, which is expected to target the full-length protein and likely recognizes all isoforms due to the conserved region. Confirm specificity by evaluating the expected band size (~76 kDa) in WB.
• For Western blot, consider using positive control lysates from heart tissue (where ZNF333 is highly expressed) and validate with siRNA knockdown or knockout samples to confirm band identity.
• In ELISA, this polyclonal antibody can be used as a capture or detection antibody; optimal coating and detection concentrations should be determined experimentally using recombinant ZNF333 protein or cell lysates.
• Cross-reactivity with mouse and rat ZNF333 has been observed; validate in the relevant sample system if using these species, as amino acid sequence similarity may affect sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for high-quality IVD raw materials, offering a comprehensive portfolio of validated antibodies, bulk ancillary reagents, and customized procurement services. This anti-ZNF333 polyclonal antibody supports research into transcriptional regulation mechanisms, making it a valuable tool for studying gene expression in cardiac and other systems. Leverage CamelBio's expertise to efficiently source rare targets, develop reliable assay components, and accelerate your IVD assay development from concept to clinic.