Anti-ZNF302 Polyclonal Antibody for WB, ELISA - Q9NR11

Core Product Specifications and Parameters

Biological Background: ZNF302 Function and Localization

• ZNF302 is a member of the zinc finger protein family, characterized by multiple C2H2-type zinc finger motifs that mediate sequence-specific DNA binding, typical of transcription factors.
• Encoded by the ZNF302 gene, this protein is also known by aliases ZNF135L, ZNF140L, and ZNF327; the gene symbol encompasses these alternative designations.
• The protein is predicted to function in transcriptional regulation, likely acting as a DNA-binding factor that modulates gene expression.
• Subcellular localization is predominantly nuclear, consistent with its role in directly interacting with genomic DNA to regulate transcription.
• Alternative splicing of the ZNF302 transcript produces multiple isoforms, expanding the functional diversity of this protein.
• Post‑translational modifications include isopeptide bond formation and potential ubiquitin‑like conjugation (based on Ubl conjugation keyword), suggesting regulation by ubiquitination or similar pathways.
• The UniProt‑computed molecular weight is 54,814 Da, closely matching the observed ~55 kDa band, with minor shifts possible due to modifications or isoform variation.

Experimental Guidance and Technical Tips

• The immunogen, spanning amino acids 90–200 of human ZNF302, encompasses part of the zinc finger region; the resulting polyclonal antibody may recognize multiple isoforms or conformational epitopes.
• For Western blot, consider starting with a standard dilution range (e.g., 1:500–1:2000) and validate band specificity using appropriate positive and negative controls (e.g., ZNF302‑overexpressing lysates or knockout samples).
• Cross‑reactivity with rat allows detection in rodent models; always confirm species‑matched reactivity by running a rat tissue or cell lysate alongside the human sample.
• In ELISA, this antibody can be employed as a capture or detection reagent; titrate the antibody concentration to optimize signal‑to‑noise ratio for your specific assay format.
• Due to alternative splicing, multiple bands may appear in Western blot; consult literature and database‑predicted isoforms to interpret the observed pattern.
• Pair this antibody with a suitable anti‑rabbit secondary and detection system appropriate for your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines access to high‑quality IVD raw materials, connecting diagnostic manufacturers and research laboratories with the reagents they need to advance from concept to clinic. This rabbit polyclonal antibody against the zinc finger transcription factor ZNF302 supports gene regulation studies and assay development. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare‑target raw materials, all backed by our commitment to helping you meet your supply requirements efficiently.