Anti-ZNF296 Rabbit Polyclonal Antibody for WB, ELISA - Q8WUU4

Core Product Specifications and Parameters

Biological Background: ZNF296 Function and Localization

• ZNF296 (Zinc Finger Protein 296), also known as ZNF342 or ZFP296, is a member of the Krüppel C2H2-type zinc finger protein family.
• It is characterized by the presence of multiple zinc finger domains, which are predicted to mediate DNA binding and transcriptional regulation.
• ZNF296 has been proposed to act as a transcriptional corepressor, potentially interacting with KLF4 (Krüppel-like factor 4).
• The protein localizes to the nucleus, consistent with its role in controlling gene expression.
• Post-translational modifications include isopeptide bonds and ubiquitin-like conjugation (Ubl conjugation), which may modulate its activity or stability.
• UniProt keywords highlight its involvement in metal binding, transcription, and nuclear localization, underscoring its importance in nuclear gene regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human ZNF296. Verify specificity by performing pre-absorption controls or using knockout samples if available.
• For Western blot (WB), the predicted molecular weight is approximately 51 kDa; however, actual migration may vary due to post-translational modifications. Use appropriate positive controls (e.g., cell lines known to express ZNF296).
• In ELISA applications, careful titration of both coating antigen and antibody is recommended to establish optimal signal-to-noise ratios.
• Given the cross-reactivity with mouse, this antibody may be suitable for comparative studies between human and mouse models; validate in the relevant species-specific sample systems.
• As a polyclonal antibody, lot-to-lot variability can occur; it is advisable to test each new batch in your assay system and set aside a reference lot for long-term consistency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of high-quality IVD raw materials for diagnostic manufacturers, laboratories, and research institutes. Our portfolio includes validated antibodies like this anti-ZNF296 polyclonal antibody for transcription factor research, complemented by bulk ancillary reagents and custom material sourcing services. Whether you need optimized antibody pairs, rare-target reagents, or end-to-end development support, CamelBio provides reliable access and expert guidance to advance your diagnostic projects.