Anti-ZNF268 (HZF3) Rabbit Polyclonal Antibody for WB, ELISA - Q14587

Core Product Specifications and Parameters

Biological Background: ZNF268 Function and Localization

• ZNF268, also known as HZF3, is a zinc finger protein that functions as a transcriptional repressor, inhibiting erythroid differentiation and tumor cell proliferation, and playing a role in ovarian cancer development and progression.
• It contributes to cervical carcinogenesis partly through the TNF-induced NF-kappa-B signaling pathway by interacting with the I-kappa-B-kinase (IKK) core complex.
• ZNF268 is involved in the regulation of antiviral interferon signaling; during viral infection, it recruits SETD4 to TBK1, leading to TBK1 monomethylation critical for TBK1 complex assembly and IRF3 signaling.
• The protein localizes to both the nucleus and cytoplasm, consistent with its roles in transcriptional regulation and signaling.
• ZNF268 exhibits tissue-specific expression: overexpressed in ovarian cancer tissues, with isoform 1 and 2 in squamous epithelium, isoform 2 overexpressed in squamous cervical cancer, and various isoforms expressed in blood cells, pancreas, lung, skeletal muscle, heart, placenta, liver, kidney, brain, and several tumor cell lines.
• The gene undergoes alternative splicing, producing multiple isoforms with distinct expression patterns.
• Key features of ZNF268 include multiple zinc finger domains, DNA-binding, metal-binding, and phosphorylation, classifying it as a reference proteome member with repressor and transcription regulation activities.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody spans amino acids 1-270, covering part of the N-terminal region; consider this when designing validation experiments.
• For Western blot (WB), the predicted molecular weight of ZNF268 is approximately 108 kDa; ensure sufficient transfer time for high-molecular-weight proteins.
• Due to cross-reactivity with human, mouse, and rat, validate the antibody in the relevant sample system, and be mindful of potential species-specific isoforms.
• In ELISA, titrate the antibody to optimize signal-to-noise ratio for your specific assay format.
• Given ZNF268’s role in cancer and viral responses, consider using positive controls from relevant tissue or cell lines (e.g., ovarian cancer, cervical cancer, or virally challenged cells) to confirm reactivity.
• As a polyclonal antibody, it may recognize multiple epitopes; verify specificity with appropriate negative controls or knockdown/knockout models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with a streamlined supply chain for IVD raw materials. This anti-ZNF268 polyclonal antibody supports research into transcriptional regulation, oncology, and antiviral innate immunity. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing solutions for rare targets, helping to accelerate your assay development from concept to clinic.