Anti-ZNF263 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O14978

Core Product Specifications and Parameters

Biological Background: ZNF263 Function and Localization

• ZNF263 is a zinc finger protein (also designated FPM315, ZSCAN44, ZKSCAN12) that contains both KRAB and SCAN domains, characteristic of a subfamily of transcriptional repressors.
• It functions as a transcription factor that binds the consensus sequence 5'-TCCTCCC-3' and represses target genes. It directly targets SIX3, recruiting chromatin modifiers to induce promoter methylation and silencing. Related references: PMID:32051553
• Additionally, ZNF263 represses HS3ST1 and HS3ST3A1 via promoter binding, further demonstrating its role in transcriptional regulation. Related references: PMID:32277030
• Subcellular localization is exclusively nuclear, consistent with its DNA-binding function.
• Broadly expressed across many tissues including heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, and leukocytes.
• Post-translational modifications include phosphorylation and isopeptide bond formation (ubiquitination); it contains multiple zinc finger motifs and binds zinc.

Experimental Guidance and Technical Tips

• The immunogen spans residues 120-240, avoiding the KRAB domain; for Western blot, expect a band near 77 kDa and consider siRNA knockdown to confirm specificity.
• For immunohistochemistry, nuclear staining is anticipated; always include a negative control lacking primary antibody and validate with known ZNF263-expressing tissues.
• This antibody shows broad species cross-reactivity (human, mouse, rat), enabling comparative studies; verify performance in your specific model system.
• ELISA utility suggests suitability for quantitative assays; consider pairing with target-specific detection reagents and optimizing coating conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers, labs, and research institutes from concept to clinic. For ZNF263-related chromatin and transcription studies, CamelBio provides validated antibody pairs, optimized polyclonal antibodies like this one, bulk ancillary reagents, and custom sourcing for rare targets. Our supply chain capabilities are designed to streamline your procurement of high-quality research and diagnostic reagents.