Anti-ZNF248 Rabbit Polyclonal Antibody for WB and ELISA - Q8NDW4

Core Product Specifications and Parameters

Biological Background: ZNF248 Function and Localization

• ZNF248 (Zinc Finger Protein 248) is a Krüppel-associated box (KRAB) domain-containing protein that belongs to the large C2H2-type zinc finger transcription factor family.
• It is predicted to act as a DNA-binding transcriptional regulator, potentially repressing target gene expression through its KRAB domain.
• The protein contains multiple C2H2 zinc finger motifs that mediate sequence-specific DNA binding.
• ZNF248 localizes to the nucleus, consistent with its role in transcriptional control.
• It undergoes post-translational modifications, including ubiquitination (Ubl conjugation) and isopeptide bond formation, which may regulate its stability or activity.
• Alternative splicing generates multiple transcript variants, possibly giving rise to functionally distinct isoforms.
• Proteomic studies have identified ZNF248 in high-throughput mass spectrometry screens, confirming endogenous expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–110) of human ZNF248. This region may target epitopes shared across splice variants, but isoform-specific reactivity should be validated in the relevant sample system.
• For Western blot, the predicted molecular weight is ~67 kDa, though actual migration may vary with post-translational modifications and gel conditions.
• This antibody is suitable for ELISA; consider pairing with a detection antibody for sandwich ELISA development and validate specificity using recombinant ZNF248 protein.
• Cross-reactivity with other species has not been tested. Before using on non-human samples, check sequence homology of the immunogen region.
• As a polyclonal antibody, it offers high avidity and tolerance to minor antigen variations, advantageous for detecting denatured or modified proteins.
• For chromatin immunoprecipitation (ChIP) or other DNA-binding applications, confirm recognition of the native protein under the intended fixation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD manufacturers and research laboratories, supporting every stage from concept to clinic. In the field of transcriptional regulation, our portfolio includes validated antibodies, recombinant proteins, and ancillary reagents to advance your diagnostic development. With robust supply chain capabilities, we ensure timely delivery of bulk raw materials, including specialized targets like ZNF248 antibodies, streamlining your procurement process.