Anti-ZNF24 Polyclonal Antibody for WB, ELISA - P17028

Core Product Specifications and Parameters

Biological Background: ZNF24 Function and Localization

• ZNF24 (Zinc finger protein 24), also known as KOX17, RSG-A, ZNF191, and ZSCAN3, is a transcription factor containing a SCAN domain and multiple C2H2-type zinc finger motifs.
• It functions as a transcriptional repressor, specifically binding to the 5'-TCAT-3' DNA motif through its zinc finger domains.
• In the central nervous system, ZNF24 is crucial for oligodendrocyte maturation: it promotes cell cycle progression in progenitor cells and is required for the transition from premyelinating to myelinating oligodendrocytes.
• Alternative splicing of ZNF24 generates multiple transcript variants, potentially contributing to functional diversity.
• The protein is subject to post-translational modifications, including phosphorylation and ubiquitination (isopeptide bond), which may regulate its activity and stability.
• ZNF24 is widely expressed in human tissues, with the notable exception of heart.
• Subcellularly, ZNF24 localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Additional keywords indicate its involvement in transcription regulation, and it has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 169-368 of human ZNF24, a region within the protein that includes part of the zinc finger domain. This suggests potential recognition of the DNA-binding domain, so consider testing the antibody in DNA-binding assays or chromatin immunoprecipitation (ChIP) if applicable.
• The predicted molecular weight is ~42 kDa (UniProt: 42155 Da). In Western blot, bands may appear near this size; however, due to post-translational modifications, slight shifts could be observed. Validate using positive control lysates from ZNF24-expressing cell lines or tissues (e.g., brain, but avoid heart).
• Cross-reactivity with mouse and rat is reported, making this antibody suitable for studies in rodent models. Optimize dilutions for each species and application.
• For ELISA, the recombinant immunogen protein can be used as a positive control or standard. Ensure proper coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive support for diagnostic manufacturers seeking high-quality raw materials, including this ZNF24 polyclonal antibody for neuroscience and transcription factor research. As a one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to streamline your assay development from concept to clinic.