Anti-ZNF213 Polyclonal Antibody for WB, IHC-P, ELISA - O14771

Core Product Specifications and Parameters

Biological Background: ZNF213 Function and Localization

• ZNF213 is a member of the krueppel C2H2-type zinc-finger protein family, characterized by the presence of KRAB (Krüppel-associated box) and SCAN domains, which are involved in protein-protein interactions and transcriptional repression.
• It may function as a transcription factor involved in the regulation of gene expression, though its specific target genes remain to be identified.
• The protein localizes to the nucleus, consistent with its role in transcriptional regulation.
• ZNF213 is widely expressed, with highest levels observed in the testis, suggesting potential roles in testicular function or spermatogenesis.
• Alternative splicing generates multiple isoforms, which may have distinct regulatory properties.
• Contains multiple C2H2 zinc finger domains that likely mediate sequence-specific DNA binding.
• Post-translational modifications such as phosphorylation and acetylation are predicted, though experimental validation is limited.
• Belongs to the ZNF family of transcription factors, which play critical roles in development, differentiation, and disease.

Experimental Guidance and Technical Tips

• The immunogen was designed using a recombinant fusion protein spanning amino acids 132-459, which encompasses the SCAN domain and several zinc finger motifs. This region is expected to generate antibodies capable of recognizing native ZNF213 in multiple assays.
• For Western blot (WB), consider using a nuclear extract as the sample source, given the protein's nuclear localization. The predicted molecular weight is ~51 kDa, but observe for potential isoform-specific bands due to alternative splicing.
• For immunohistochemistry (IHC-P), perform antigen retrieval as the antibody targets an intracellular protein. Validate the staining pattern by comparing with known markers or negative controls.
• In ELISA, titrate the antibody to determine optimal coating concentrations and detection ranges; cross-reactivity with mouse and rat ZNF213 should be verified experimentally.
• This antibody has been reported to react with human, mouse, and rat samples, but homology of the immunogen sequence should be checked for other species if broader cross-reactivity is desired.
• For critical experiments, we recommend including appropriate positive and negative controls, such as siRNA-mediated knockdown or overexpression cell lines, to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, offering a wide range of high-quality antibodies, antigens, and ancillary reagents. This anti-ZNF213 polyclonal antibody is part of our transcription factor portfolio, supporting research into gene regulation and biomarker discovery. Whether you need bulk quantities, custom conjugations, or complementary reagents for immunoassay development, CamelBio streamlines your supply chain from discovery to commercialization.