Anti-ZNF207 (BuGZ, hBuGZ) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O43670

Core Product Specifications and Parameters

Biological Background: ZNF207 Function and Localization

• ZNF207, also known as BuGZ or hBuGZ, is a zinc finger protein that interacts with BUB3 and contains a GLEBS motif. It belongs to the zinc finger family and exists in alternatively spliced isoforms.
• It functions as a kinetochore- and microtubule-binding protein essential for spindle assembly. ZNF207 is largely composed of disordered low-complexity regions and can undergo phase transition to form liquid droplets, which promotes microtubule bundling, concentrates tubulin, and facilitates spindle matrix formation. Related references: PMID:24462186, PMID:24462187, PMID:26388440
• ZNF207 regulates mitotic chromosome alignment by stabilizing BUB3 and promoting its loading onto kinetochores. One proposed mechanism involves blocking BUB3 ubiquitination and proteasomal degradation, while another study indicates proteasome-independent stabilization. Related references: PMID:24462186, PMID:24462187
• Subcellularly, ZNF207 localizes to the nucleus, specifically at the chromosome centromere and kinetochore, and also associates with the spindle and cytoplasmic microtubules during mitosis.
• The protein is ubiquitously expressed across human tissues, consistent with its fundamental role in cell division.
• ZNF207 contains zinc-finger domains and is classified as a DNA-binding, metal-binding protein; it participates in cell cycle, cell division, mitosis, and chromosome partition processes.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-85 of human ZNF207 (NP_003448.1), providing targeting of the N-terminal region. Researchers should validate the antibody’s performance in their specific experimental context.
• For Western blot (WB), consider using whole-cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is approximately 51 kDa. Ensure appropriate gel percentage and loading controls.
• For immunohistochemistry (IHC-P), suitable positive control tissues include those with high mitotic index; optimization of antigen retrieval and antibody dilution may be necessary.
• In ELISA, use recombinant ZNF207 protein or peptide as coating antigen, and perform cross-reactivity testing if needed.
• Given the polyclonal nature, lot-to-lot consistency should be verified, especially for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This anti-ZNF207 antibody supports research in cell division, mitosis, and kinetochore biology—aligning with diagnostic development needs. Whatever your development pipeline requires, CamelBio bridges the gap from concept to clinic with reliable supply and technical support.