Anti-ZNF20 Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA - P17024

Core Product Specifications and Parameters

Biological Background: ZNF20 Function and Localization

• ZNF20 (Zinc Finger Protein 20, alias KOX13) is a human zinc finger transcription factor encoded by the ZNF20 gene.
• It belongs to the C2H2-type zinc finger protein family and contains multiple zinc finger repeats that facilitate DNA binding.
• ZNF20 may be involved in transcriptional regulation, as suggested by its functional annotation.
• The protein is localized to the nucleus, consistent with its role in gene expression control.
• According to UniProt, the calculated molecular weight is ~61.6 kDa, while experimentally it migrates at approximately 62 kDa.
• This target is classified under keywords such as DNA-binding, metal-binding, nucleus, transcription regulation, and zinc-finger.
• No tissue-specific expression patterns or antimicrobial functions have been reported for ZNF20.
• The lack of PubMed references in the UniProt record indicates that ZNF20 remains understudied, presenting opportunities for basic research.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within the N-terminal portion of human ZNF20 (amino acids 51–118), which may confer high specificity for the target.
• For Western blotting, a band around 62 kDa is anticipated; consider using nuclear extracts or whole-cell lysates from human, mouse, or rat cells.
• In immunofluorescence assays (IF/ICC, IF-P), a predominantly nuclear signal is expected; validate with appropriate organelle markers.
• The antibody's cross-reactivity with mouse and rat suggests conserved epitope sequences; however, confirm reactivity in the species and tissue of interest.
• As a rabbit monoclonal antibody, it may provide batch-to-batch consistency; optimize blocking and detection conditions for best results.
• For ELISA, titrate the antibody and include a negative control to assess background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-ZNF20 monoclonal antibody supports studies on zinc finger-mediated transcriptional regulation. Beyond antibodies, we offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets to accelerate your IVD development.