Anti-ZNF195 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O14628

Core Product Specifications and Parameters

Biological Background: ZNF195 Function and Localization

• Zinc finger protein 195 (ZNF195) is a zinc-finger domain-containing protein that belongs to a family of transcription factors. It may be involved in transcriptional regulation.
• The protein localizes to the nucleus, consistent with its putative role in DNA binding and gene expression control.
• Tissue expression analysis reveals ZNF195 in adult heart, brain, placenta, skeletal muscle, and pancreas, with lower levels in adult lung, liver, and kidney. It is also detected in fetal lung, kidney, and brain.
• Post-translational modifications such as acetylation and ubiquitination (isopeptide bond) are indicated, potentially modulating protein function or stability.
• Alternative splicing generates multiple isoforms, which may contribute to functional diversity.
• UniProt keywords include metal-binding and zinc-finger repeats, highlighting its structural features as a zinc-binding protein.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-250 (N-terminal region) of human ZNF195 (NP_001123991.1). Consider this when designing epitope-specific experiments.
• For Western blotting, the expected molecular weight is approximately 72 kDa (UniProt: 72.3 kDa). Optimize gel percentage and transfer conditions accordingly.
• For IF/ICC, anticipate nuclear staining. Use appropriate permeabilization and fixation protocols to ensure antibody access.
• For ELISA, perform standard checkerboard titration of the antibody and antigen to determine optimal working concentrations.
• Reported cross-reactivity with mouse and rat suggests conservation of the immunogenic sequence; however, validation in your sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing diagnostic manufacturers and research labs with access to validated antibodies, bulk reagents, and technical support from concept to clinic. For transcription factor research involving ZNF195, we offer a range of high-quality polyclonal antibodies, ancillary reagents, and supply chain solutions tailored to your immunoassay development needs.