Anti-ZNF182 Polyclonal Antibody for WB and ELISA - P17025

Core Product Specifications and Parameters

Biological Background: ZNF182 Function and Localization

• ZNF182, also known as Zinc finger protein 21 or KOX14, belongs to the krueppel C2H2-type zinc-finger protein family.
• It likely functions as a transcriptional regulator, binding DNA through its conserved zinc-finger motifs to modulate gene expression.
• The protein localizes to the nucleus, consistent with its predicted role in transcriptional control.
• ZNF182 contains multiple C2H2-type zinc-finger domains, which are characteristic of sequence-specific DNA-binding proteins.
• Alternative splicing of ZNF182 transcripts may lead to distinct protein isoforms with potentially different regulatory properties.
• Keywords associated with ZNF182 include DNA-binding, Metal-binding, Transcription regulation, and Zinc-finger, underscoring its role as a nuclear transcription factor.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody maps to a region within the N-terminal part of human ZNF182 (residues 100-210). This region may be conserved across species, consistent with its cross-reactivity with mouse and rat homologs.
• For Western blot, using a whole-cell extract from human, mouse, or rat cell lines is recommended. The expected molecular weight is approximately 74 kDa; however, due to potential alternative splicing or post-translational modifications, additional bands may be observed.
• In ELISA applications, the recombinant protein or peptide corresponding to the immunogen can be used as a positive control; titration of both coating antigen and primary antibody is advised for optimal signal-to-noise ratio.
• Since ZNF182 is a transcription factor with low endogenous expression in many cell types, consider using nuclear-enriched lysates or overexpressed samples to improve detection sensitivity.
• Validation of antibody specificity can be performed by siRNA knockdown or overexpression experiments in cell lines expressing ZNF182.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. For research on zinc finger transcriptional regulators like ZNF182, CamelBio can support your assay development needs from concept to clinic with reliable supply chain and technical expertise.