Anti-ZNF177 Rabbit Polyclonal Antibody for WB, ELISA - Q13360

Core Product Specifications and Parameters

Biological Background: ZNF177 Function and Localization

• ZNF177 is a zinc finger protein containing C2H2-type zinc finger domains, which are classical DNA-binding motifs. It is predicted to function as a transcription factor that may regulate gene expression.
• The protein is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.
• ZNF177 undergoes alternative splicing, producing multiple transcript variants with potentially distinct functional roles.
• The protein contains metal-binding zinc finger repeats, contributing to its structural stability and DNA-binding specificity.
• As a member of the zinc finger protein family, ZNF177 likely participates in chromatin remodeling and transcriptional control of target genes.
• UniProt lists proteomics identification as a keyword, indicating its detection in high-throughput protein expression studies.
• The calculated molecular weight is 54.8 kDa, while the observed band may appear at ~55 kDa in western blotting.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering the N-terminal region (aa 50-110). Consider using this sequence information to verify epitope specificity.
• For western blot, a band of ~55 kDa is expected. Validate antibody specificity using positive control lysates from human or mouse cells known to express ZNF177.
• ELISA can be performed using the same recombinant protein as coating antigen. Optimization of antibody dilution is recommended.
• Note cross-reactivity with mouse ZNF177; perform species-specific validation when using mouse tissues or cell lysates.
• Store antibody at recommended temperature and avoid repeated freeze-thaw cycles to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement for transcriptional regulation targets like ZNF177. We connect diagnostic manufacturers and research labs with reliable, high-quality reagents to streamline assay development from concept to clinic.