Anti-ZNF177 Rabbit Polyclonal Antibody for IHC-P, IF/ICC, ELISA - Q13360

Core Product Specifications and Parameters

Biological Background: ZNF177 Function and Localization

• ZNF177 belongs to the zinc finger protein family, characterized by C2H2-type zinc finger domains that mediate DNA binding.
• It is predicted to function as a transcription factor, potentially regulating gene expression through sequence-specific DNA interactions.
• ZNF177 localizes to the nucleus, consistent with its role in transcriptional regulation.
• The protein may undergo alternative splicing, producing multiple transcript variants.
• Zinc finger proteins like ZNF177 often contain multiple tandem zinc finger repeats that confer binding specificity.
• ZNF177 has been identified in proteomics studies, indicating its expression in human cells.
• The molecular weight of ZNF177 is approximately 55 kDa, as determined by its amino acid sequence.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 50-110 of human ZNF177; consider sequence alignment with mouse and rat orthologs to confirm epitope conservation for cross-reactivity.
• For IHC-P, validate in appropriate tissue types known to express ZNF177 using positive and negative controls.
• For IF/ICC, ensure appropriate fixation and permeabilization methods to detect nuclear localization.
• ELISA can be used for quantitative detection; optimize antibody dilution in your sample system.
• Since ZNF177 is a transcription factor, consider testing in nuclear extracts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined pathway to IVD raw materials, technical expertise, and consulting—from early development through clinical application. As a single-source partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support transcriptional regulation research and beyond. Let CamelBio connect your project with the right raw materials efficiently and reliably.