Anti-ZNF143 Rabbit Polyclonal Antibody (WB, ELISA) - P52747

Core Product Specifications and Parameters

Biological Background: ZNF143 Function and Localization

• ZNF143, also known as SPH-binding factor (SBF) and selenocysteine tRNA gene transcription-activating factor (hStaf), is a zinc finger transcription factor encoded by the ZNF143 gene in humans.
• It functions as a transcriptional activator for genes transcribed by both RNA polymerase II and RNA polymerase III, playing a crucial role in maintaining mitochondrial activity. Related references: PMID:10893243 PMID:14702349 PMID:17938208
• ZNF143 specifically binds to the Staf-binding site (SBS), a consensus DNA motif present in thousands of gene promoters, thereby regulating a broad transcriptional network. Related references: PMID:17092945 PMID:21177654 PMID:23408857
• It is essential for the expression of selenocysteine tRNA (tRNAsec), small nuclear RNAs (snRNAs), and a subset of mRNAs that control cell proliferation and differentiation. Related references: PMID:23152058 PMID:17938208 PMID:9776743
• The protein localizes to the nucleus and associates with chromosomes, consistent with its role in transcriptional regulation.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin conjugation, which may modulate its activity and stability, as suggested by proteomics identification.
• Through its regulatory network, ZNF143 contributes to the maintenance of embryonic stem cells by facilitating the binding of POU5F1/OCT4 to target promoters, ultimately promoting NANOG expression.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 437–638 of human ZNF143; consider that the polyclonal antibody may recognize additional epitopes beyond the immunogen region.
• For WB, a band around 69 kDa is expected in lysates from human, mouse, and rat samples; validate specificity with appropriate controls.
• Perform an initial titration experiment to determine the optimal primary antibody dilution for your specific experimental setup.
• Since cross-reactivity is reported for mouse and rat, the antibody can be used in comparative studies across these species, but independent confirmation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, research laboratories, and biotech companies from concept to clinic. This anti-ZNF143 antibody serves as a key reagent for investigating transcriptional regulation and mitochondrial research. Whether you need validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, or rare target raw-material sourcing, CamelBio offers integrated supply solutions to accelerate your development pipeline.