Anti-ZNF133 Rabbit Polyclonal Antibody for WB, ELISA - P52736

Core Product Specifications and Parameters

Biological Background: ZNF133 Function and Localization

• ZNF133 (Zinc Finger Protein 133), also known as ZNF150, is a member of the C2H2-type zinc finger family, characterized by multiple zinc finger domains that mediate DNA binding.
• Functions as a transcriptional repressor, likely regulating gene expression by binding to specific DNA sequences in target promoters.
• Localizes predominantly to the nucleus, consistent with its role in transcription regulation.
• Widely expressed across multiple tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas, suggesting a general regulatory role.
• The ZNF133 gene undergoes alternative splicing, potentially producing multiple protein isoforms with distinct functional properties (Keywords: Alternative splicing).
• Contains isopeptide bonds and sites for ubiquitin-like conjugation (Ubl), indicating regulation by post-translational modifications such as ubiquitination or sumoylation.
• Zinc-finger motifs coordinate zinc ions (metal-binding) essential for structural integrity and DNA-binding activity.

Experimental Guidance and Technical Tips

• The immunogen (aa 1-220, N-terminal region) may allow recognition of multiple isoforms if they share this region; validate in your specific experimental system.
• For Western blot, consider using whole-cell lysates from human, mouse, or rat tissues/cell lines, as ZNF133 is ubiquitously expressed. The predicted molecular weight is 73 kDa.
• For ELISA, the recombinant immunogen can serve as a positive control to establish standard curves and assess sensitivity.
• Include appropriate reducing agents during sample preparation to ensure complete denaturation of zinc-finger-containing proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for IVD raw materials, offering a broad spectrum of high-quality antibodies, antigens, and ancillary reagents to support diagnostic development from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk reagent supply, complemented by technical and consulting services. For transcription regulation research and assay development targeting ZNF133, we can facilitate sourcing of the required raw materials through our integrated supply chain.