Anti-ZNF10 Polyclonal Antibody for WB, ELISA - P21506

Core Product Specifications and Parameters

Biological Background: ZNF10 Function and Localization

• Zinc finger protein 10 (ZNF10), also known as zinc finger protein KOX1, is a member of the Krüppel-associated box (KRAB) domain-containing zinc finger protein family, characterized by C2H2-type zinc finger motifs that typically mediate sequence-specific DNA binding.
• Encoded by the ZNF10 gene (human GeneID: 7556), this protein is classified as a putative transcription factor, though its direct target genes and precise role in gene regulation remain less defined compared to other family members.
• FUNCTION: May be involved in transcriptional regulation, suggesting a role in modulating gene expression, potentially through KRAB domain-mediated repression or zinc finger-directed DNA interactions.
• SUBCELLULAR LOCATION: Localized to the nucleus, consistent with its predicted DNA-binding and regulatory functions; nuclear compartmentalization is essential for its activity.
• The protein contains multiple tandem zinc finger repeats and is associated with keywords including DNA-binding, Metal-binding (zinc), Nucleus, Transcription, and Transcription regulation, indicating its involvement in chromatin and gene control pathways.
• UniProt annotates it under the Reference proteome set and as a proteomics-identified target, highlighting its experimental verification in large-scale human proteome studies.
• No tissue-specific expression or antimicrobial roles are reported for ZNF10 in the available records, making its functional specificity an active research area.

Experimental Guidance and Technical Tips

• The immunogen used to generate this rabbit polyclonal antibody spans amino acids 474–573 (NP_056209.2), a C-terminal region that may offer isoform specificity; researchers should verify cross-reactivity with other ZNF isoforms in their model system using knockdown or knockout controls.
• For Western blot (WB), expect a band near 66 kDa (UniProt calculated mass: 66,455 Da); due to the nuclear localization of ZNF10, consider using nuclear fractionation or high-salt extraction protocols to enhance detection and reduce background.
• In ELISA applications, this polyclonal antibody may be used for direct or indirect detection; optimization of coating antigen concentration and antibody dilution is recommended based on the target protein's availability and purity.
• Cross-reactivity is reported for human ZNF10; for experiments involving other species, consult protein sequence alignment tools (e.g., BLAST) to assess homology and potential cross-reactivity before use.
• No pre-determined dilutions or quantitative performance data are provided; pilot titration is advised across a range of concentrations (e.g., 1:500–1:5000 for WB) to determine optimal conditions.

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As a dedicated partner for IVD manufacturers, CamelBio simplifies raw-material procurement by offering this anti-ZNF10 polyclonal antibody for transcription factor research, along with a broad portfolio of validated antibodies, optimized recombinant proteins, and bulk ancillary reagents. Whether you need monoclonal antibody pairs, highly specific polyclonal antibodies, or assistance with rare-target sourcing, our one-stop supply chain covers every stage from concept to clinic. Connect with us to streamline your diagnostic development with reliable, research-ready materials.