Anti-ZMYND8 Polyclonal Antibody for WB, ELISA - Q9ULU4

Core Product Specifications and Parameters

Biological Background: ZMYND8 Function and Localization

• ZMYND8 (MYND-type zinc finger-containing chromatin reader) is a member of the zinc finger protein family and serves as a reader of dual histone modifications, recognizing marks such as H3.1K36me2-H4K16ac and H3K4me1-H3K14ac. This allows it to act as a transcriptional corepressor for histone demethylases KDM5D, KDM5C, and EZH2. Related references: PMID:26655721 PMID:27477906 PMID:31965980 PMID:33323928
• In response to DNA damage, ZMYND8 recognizes acetylated histone H4 and recruits the NuRD chromatin remodeling complex to damaged chromatin, facilitating transcriptional repression and double-strand break repair by homologous recombination. Related references: PMID:25593309 PMID:27732854 PMID:30134174
• ZMYND8 also activates transcription elongation by RNA polymerase II through recruitment of the P-TEFb complex to target promoters, particularly at all-trans-retinoic acid (ATRA)-responsive genes, thereby positively regulating their expression. Related references: PMID:26655721 PMID:30134174
• It promotes neuronal differentiation by associating with regulatory regions of the MAPT gene, enhancing transcription of a protein-coding MAPT isoform while suppressing the non-coding MAPT213 isoform. Related references: PMID:30134174 PMID:35916866 PMID:36064715
• ZMYND8 functions as a tumor suppressor, suppressing breast and prostate cancer cell invasion and metastasis. Related references: PMID:27477906 PMID:31965980 PMID:33323928
• Subcellularly, ZMYND8 localizes predominantly to the nucleus and chromosomes, with some presence in the cytoplasm.
• The protein is expressed in a wide range of tissues, with highest levels in brain, lung, pancreas, and placenta; at the protein level, it is detected in neurons but absent in astrocytes.
• ZMYND8 is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may regulate its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen, a recombinant protein covering amino acids 959-1188 of human ZMYND8, includes the C-terminal region that may contain functional domains involved in chromatin binding. Consider this when designing validation experiments.
• For Western blotting, the predicted molecular weight of ZMYND8 is approximately 132 kDa. Optimize protein extraction and transfer conditions for high-molecular-weight proteins.
• This antibody is recommended for ELISA; ensure proper antigen coating and antibody dilution for specific detection in human samples.
• Validate antibody specificity using appropriate controls, such as knockout cell lines or siRNA-mediated knockdown, especially in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop partner for diagnostic manufacturers and research laboratories, offering end-to-end access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-ZMYND8 polyclonal antibody supports advanced research in epigenetics, transcriptional regulation, and cancer biology. We supply validated antibody pairs, bulk ancillary reagents, and custom rare-target sourcing to accelerate your assay development and commercialization.