Anti-ZMPSTE24 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75844

Core Product Specifications and Parameters

Biological Background: ZMPSTE24 Function and Localization

• ZMPSTE24, also known as CAAX prenyl protease 1 homolog or FACE-1, is a zinc metalloprotease essential for the maturation of lamin A and other farnesylated proteins.
• It proteolytically removes the C-terminal three residues of farnesylated proteins, playing a critical role in nuclear envelope organization and protein homeostasis. Related references: PMID:33293369, PMID:33315887
• ZMPSTE24 localizes to the endoplasmic reticulum and nuclear inner membranes, as well as to early and late endosomal membranes, reflecting its diverse cellular roles.
• Beyond its protease activity, ZMPSTE24 functions as a broad‑spectrum antiviral factor by restricting entry of enveloped viruses such as influenza A, Zika, Ebola, and others, partially through modulation of membrane fluidity and the IFITM pathway. Related references: PMID:28169297, PMID:28246125, PMID:35283811
• The protein is widely expressed, with particularly high levels in kidney, prostate, testis, and ovary, indicating tissue‑specific regulatory functions.
• ZMPSTE24 is associated with disease variants, including progeroid syndromes, underscoring its clinical importance.

Experimental Guidance and Technical Tips

• For Western blotting, a band near 55 kDa is expected; validation in endogenously expressing cell lines or tissues (e.g., kidney, testis) is recommended.
• Immunohistochemistry (IHC‑P) may require optimization of antigen retrieval and antibody dilution; positivity should be verified in tissues with known ZMPSTE24 expression.
• The antibody is predicted to cross‑react with mouse and rat ZMPSTE24, making it suitable for studies in rodent models; however, sequence alignment of the immunogen region is advised to confirm reactivity.
• ELISA performance should be evaluated against a defined antigen (e.g., recombinant ZMPSTE24 or peptide) under standardized conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner, providing comprehensive access to validated antibodies, bulk ancillary reagents, and rare‑target sourcing for diagnostic manufacturers and research laboratories. This anti‑ZMPSTE24 polyclonal antibody supports investigation into laminopathies, antiviral innate immunity, and nuclear envelope dynamics. Through a global network and rigorous quality management, CamelBio streamlines procurement—from single‑clone characterization to large‑scale production—helping you accelerate assay development and translational research.