Anti-ZMIZ2 Rabbit Polyclonal Antibody for WB and ELISA - Q8NF64

Core Product Specifications and Parameters

Biological Background: ZMIZ2 Function and Localization

• ZMIZ2, also known as PIAS-like protein Zimp7, is a zinc finger MIZ domain-containing protein that structurally resembles members of the PIAS (protein inhibitor of activated STAT) family, suggesting a potential role in SUMOylation pathways.
• It functions as a coactivator that enhances the ligand-dependent transcriptional activity of the androgen receptor (AR) and other nuclear hormone receptors.
• The protein is predominantly localized in the nucleus, consistent with its role in transcriptional regulation.
• ZMIZ2 exhibits highest expression in testis, with lower levels detected in heart, brain, pancreas, prostate, and ovary.
• Post-translational modifications include isopeptide bond formation (indicative of ubiquitin-like conjugation) and methylation, as annotated in UniProt.
• The presence of zinc‑finger domains enables zinc ion binding, which is critical for protein structure and interaction with transcriptional machinery.
• Alternative splicing generates multiple isoforms, potentially expanding functional diversity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1–110) of human ZMIZ2; this region is likely conserved among the reported reactive species (human, mouse, rat) and may recognize different isoforms if the epitope is present in all variants.
• For Western blot, a band around 97 kDa is expected. Testis lysate is recommended as a positive control given the high expression of ZMIZ2 in this tissue.
• In ELISA applications, titrate the antibody and optimize coating antigen concentration to achieve the desired sensitivity and specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports researchers and diagnostic developers with comprehensive IVD raw‑material solutions, including validated antibodies for nuclear receptor and transcription research, such as this anti‑ZMIZ2 polyclonal antibody. Our supply capabilities encompass optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your workflow from early development to regulatory approval.