Anti-ZIP8 Rabbit Polyclonal Antibody for WB and ELISA - Q9C0K1

Core Product Specifications and Parameters

Biological Background: ZIP8 Function and Localization

• ZIP8, encoded by SLC39A8, is a member of the LIV-1 subfamily of ZIP zinc transporters and functions as an electroneutral divalent metal cation/bicarbonate symporter for zinc, manganese, iron, cobalt, and cadmium (PubMed:12504855, PubMed:22898811, PubMed:23403290, PubMed:27166256, PubMed:31699897, PubMed:27466201). Related references: PMID:12504855 PMID:22898811 PMID:23403290 PMID:27166256 PMID:31699897 PMID:27466201

• It plays a critical role in innate and adaptive immunity by regulating zinc influx, modulating monocyte adhesion, and controlling inflammatory gene expression (PubMed:18390834, PubMed:23403290, PubMed:28056086). Related references: PMID:18390834 PMID:23403290 PMID:28056086

• ZIP8 is essential for manganese homeostasis, recovering the metal from bile in hepatocytes, reabsorbing it in kidney proximal tubules, and facilitating its transport across the blood-brain barrier (PubMed:26637978, PubMed:28481222, PubMed:31699897). Related references: PMID:26637978 PMID:28481222 PMID:31699897

• The protein localizes to the cell membrane, lysosomes, and both apical and basolateral membranes, enabling nutrient uptake and intracellular zinc mobilization (PubMed:19401385). Related references: PMID:19401385

• Alternatively spliced isoforms, glycosylation, and signal peptide processing contribute to ZIP8 regulation; mutations are linked to a congenital disorder of glycosylation (CDG2N).

• ZIP8 indirectly regulates transcription factors such as MTF1 and influences expression of metalloproteases and cytoskeleton-related proteins (PubMed:29337306, PubMed:29927450). Related references: PMID:29337306 PMID:29927450

• It also participates in selenium uptake by transporting selenite, highlighting its broader micronutrient transport capacity (PubMed:27166256). Related references: PMID:27166256

• Tissue expression is highest in pancreas, with significant levels in thymus, placenta, lung, liver, and immune cells such as macrophages (PubMed:12504855, PubMed:22898811, PubMed:28056086, PubMed:31699897). Related references: PMID:12504855 PMID:22898811 PMID:28056086 PMID:31699897

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 201–300) lies within an extracellular loop, which may enhance recognition of native ZIP8. For WB, consider testing a range of reducing and non-reducing conditions given the multiple transmembrane domains.
• Validate specificity in the relevant sample system using knockout controls or peptide blocking, especially when analyzing tissues with high endogenous peroxidase activity.
• For ELISA applications, pairing with a monoclonal capture antibody may improve sandwich assay performance due to the polyclonal nature of the detection antibody.
• Cross-reactivity with mouse and rat ZIP8 is reported; verify sequence homology in the immunogen region if using these species, and optimize detection conditions accordingly.
• Monitor protein stability, as ZIP8 is a glycosylated membrane protein; include protease inhibitors and avoid boiling samples to prevent aggregation.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw material provider, CamelBio supports diagnostic manufacturers and research laboratories with access to validated antibodies, bulk ancillary reagents, and custom sourcing for zinc transporter targets like ZIP8. Whether you require optimized polyclonal antibodies for WB/ELISA, complementary monoclonal pairs, or rare target raw materials, CamelBio streamlines your procurement from concept to clinic. Contact us to explore how we can accelerate your immunoassay development with reliable ZIP8 antibody supplies.