Anti-ZIP14 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15043

Core Product Specifications and Parameters

Biological Background: ZIP14 Function and Localization

• ZIP14, encoded by the SLC39A14 gene, is a ubiquitously expressed metal cation symporter belonging to the LIV-1 subfamily of ZIP zinc transporters (also known as LZT-Hs4). It mediates cellular uptake of zinc, manganese, and iron, which are vital for tissue homeostasis, metabolism, development, and immunity. Related references: PMID:15642354, PMID:27231142, PMID:29621230
• It functions as an electroneutral transporter, moving a divalent metal cation together with two bicarbonate anions across the plasma membrane. In addition to endogenous metals, ZIP14 can also import the toxic metal cadmium.
• ZIP14 plays a critical role in systemic manganese homeostasis by mediating basolateral manganese uptake in intestinal epithelial cells and uptake at the blood-brain barrier. Related references: PMID:31028174, PMID:31699897
• The protein is localized to the plasma membrane (apical and basolateral domains) as well as to early endosome, late endosome, and lysosome membranes, consistent with its roles in both cell-surface uptake and intracellular trafficking of metals.
• ZIP14 is involved in zinc-dependent regulation of insulin receptor signaling in hepatocytes, influencing glucose uptake, glycogen synthesis, and gluconeogenesis through the catabolism of insulin. Related references: PMID:27703010
• In macrophages, ZIP14 modulates innate immune responses by regulating cytokine expression. Related references: PMID:23052185
• ZIP14 is broadly expressed, with notably high levels in liver, pancreas, and thyroid, and is also found in fetal tissues, brain large neurons, osteoblasts, macrophages, and blood-brain barrier endothelial cells. Related references: PMID:15642354, PMID:20682781, PMID:7584044, PMID:27231142, PMID:29621230, PMID:31699897, PMID:23052185
• Alternative splicing produces multiple isoforms; the antibody was raised against a synthetic peptide within residues 101-200 of human ZIP14 isoform 1 (NP_001121903.1).

Experimental Guidance and Technical Tips

• For Western blotting, a band around 54 kDa is predicted under reducing conditions; validation in the relevant sample system is recommended.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval may be necessary; consider testing known positive tissues such as liver or pancreas.
• The antibody cross-reacts with mouse and rat; confirm sequence homology of the immunogen region in these species before use in rodent models.
• For ELISA applications, proper coating conditions and detection systems may require optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, supporting research into metal ion transport and zinc homeostasis with high-quality antibodies like this anti-ZIP14 polyclonal antibody. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing for rare targets—helping diagnostic manufacturers and researchers streamline their workflow from concept to clinic.