Anti-ZIP12 Rabbit Polyclonal Antibody for WB, ELISA - Q504Y0

Core Product Specifications and Parameters

Biological Background: ZIP12 Function and Localization

• ZIP12 (SLC39A12) is a zinc transporter that mediates Zn²⁺ import across the plasma membrane, functioning as a uniporter with temperature-dependent activity.
• It is a member of the LIV-1 subfamily of ZIP zinc transporters (also known as LZT-Hs8), characterized by multiple transmembrane helices.
• ZIP12 may contribute to early developmental processes, including neurulation and neurite extension.
• It plays a potential role in spermatogenesis by maintaining intracellular zinc levels that reduce the inhibitory effects of oxidative stress on spermatogonia and spermatozoa viability.
• The protein is localized to the cell membrane and is expressed predominantly in the brain and eye, with alternative splicing isoforms reported.
• Keywords include: Alternative splicing, Ion transport, Membrane, Transmembrane helix, Zinc transport.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100) of human ZIP12. For increased specificity in western blot, consider using the immunizing peptide to pre-absorb the antibody.
• This antibody is suitable for western blot (WB) and ELISA applications. For WB, the predicted molecular weight is 77 kDa, but post-translational modifications or alternative isoforms may result in multiple bands; ensure appropriate sample preparation and controls.
• Cross-reactivity with mouse and rat is reported, which may allow studies in rodent models. Validate dilution factors and blocking conditions in the relevant species and tissue (e.g., brain, eye, or testis).
• Given the transporter function, consider using zinc supplementation or deprivation experiments to explore target dynamics.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with one-stop access to IVD raw materials, including validated antibodies like the anti-ZIP12 polyclonal antibody. Beyond individual reagents, we support bulk procurement of antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for zinc transporter studies. Whether you require rare target raw-material sourcing or a complete supply chain solution, CamelBio bridges your concept-to-clinic pipeline with reliable B2B service.