Anti-Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit Polyclonal Antibody for WB, ELISA - P25311

Core Product Specifications and Parameters

Biological Background: Zinc-α2-glycoprotein Function and Localization

• Zinc-α2-glycoprotein (AZGP1, also designated ZAG or ZA2G) is a secreted glycoprotein with a calculated molecular weight of ~34.2 kDa.
• It stimulates lipid degradation in adipocytes and is implicated in the extensive fat losses associated with advanced cancers (cancer cachexia).
• ZAG may bind polyunsaturated fatty acids, suggesting a potential role in lipid transport or metabolism.
• The protein is present in multiple body fluids and tissues, including blood plasma, seminal plasma, urine, saliva, sweat, liver, and epithelial cells of secretory glands.
• Subcellularly, it is secreted into the extracellular space.
• Post-translational modifications include glycosylation and a pyrrolidone carboxylic acid at the N-terminus; structural features involve disulfide bonds and a solved 3D structure.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 21–298, spanning the majority of the mature protein; this design typically elicits antibodies recognizing multiple epitopes.
• For WB, a band near 34 kDa is anticipated; consider using reducing conditions and validating in relevant samples such as serum, plasma, or lysates from liver or adipose tissue.
• ELISA applications may benefit from paired antibody screening to develop robust sandwich assays; cross-reactivity with mouse ZAG allows for translational studies.
• Since this is a polyclonal antibody, lot-to-lot consistency should be monitored; always include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-ZAG polyclonal antibody as part of a complete IVD raw-material portfolio, including validated antibody pairs, bulk ancillary reagents, and optimized polyclonal/monoclonal antibodies for diagnostic manufacturing. Our one-stop sourcing model helps you accelerate metabolic and oncology assay development, and our team is ready to assist with rare-target raw-material procurement from concept to clinic.