Anti-Zinc-α2-glycoprotein (ZAG/AZGP1) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P25311

Core Product Specifications and Parameters

Biological Background: Zinc-α2-glycoprotein Function and Localization

• Zinc-α2-glycoprotein (ZAG), encoded by the AZGP1 gene, is a secreted protein also known as ZNGP1.
• It stimulates lipid degradation in adipocytes and is implicated in the extensive fat loss (cachexia) associated with advanced cancers.
• ZAG may bind polyunsaturated fatty acids, suggesting a role in lipid transport or metabolism.
• The protein is found in various body fluids including blood plasma, seminal plasma, urine, saliva, and sweat, and is secreted by epithelial cells of multiple glands and the liver.
• It is a secreted glycoprotein with a signal peptide, confirmed by proteomics identification and direct protein sequencing.
• Structural features include a disulfide bond and a pyrrolidone carboxylic acid modification, characteristic of secreted proteins.
• The UniProt record indicates a molecular weight of about 34 kDa, consistent with a single-chain glycoprotein.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 1-100 of human AZGP1 (P25311). This region includes the signal peptide and N-terminal domain, which may allow recognition of both the precursor and mature secreted forms.
• For Western blot (WB), consider using serum or cell culture supernatant samples, and expect a band near 34 kDa under reducing or non-reducing conditions.
• For immunohistochemistry (IHC-P), staining of secretory epithelial tissues (e.g., liver, salivary glands) is anticipated; optimization of antigen retrieval may improve signal.
• For ELISA, the antibody can potentially be used as a detection or capture reagent for quantifying soluble ZAG in biological fluids; validation with a matched antibody pair is recommended.
• Experimental conditions such as antibody dilution, incubation time, and blocking buffer should be optimized in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, offering validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and research labs from concept to clinic. For studies on lipid metabolism, cancer cachexia, and secreted biomarkers like AZGP1, we provide comprehensive raw material solutions tailored to your assay development needs.