Anti-ZHX2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9Y6X8

Core Product Specifications and Parameters

Biological Background: ZHX2 Function and Localization

• ZHX2 functions as a transcriptional repressor, directly binding to DNA and regulating gene expression. Related references: PMID:12741956
• It represses the promoter activity of the CDC25C gene stimulated by NFYA, indicating a role in cell cycle regulation. Related references: PMID:12741956
• ZHX2 is ubiquitously expressed and detected in podocytes, suggesting a broad regulatory role.
• The protein is localized to the nucleus, consistent with its function as a transcription factor.
• ZHX2 may play a role in retinal development by regulating the composition of bipolar cell populations, promoting differentiation of bipolar OFF-type cells.
• In the brain, ZHX2 may promote maintenance and suppress differentiation of neural progenitor cells in the developing cortex.
• ZHX2 contains multiple zinc-finger domains and a homeobox domain, enabling DNA binding and transcriptional regulation.
• Post-translational modifications include phosphorylation and ubiquitination, which may modulate its activity and stability.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein spanning amino acids 488–837 of human ZHX2, which includes the zinc-finger and homeobox domains. It may recognize both full-length and potential isoforms containing this region.
• For Western blot (WB), anticipate a band at approximately 92 kDa corresponding to the full-length ZHX2 protein. Verify specificity using positive and negative controls, and consider optimizing blocking and antibody dilution conditions.
• The antibody is suitable for immunohistochemistry (IHC-P) and ELISA. For IHC, ensure proper antigen retrieval and validate in tissues known to express ZHX2 (e.g., retina, brain, podocyte-rich kidney sections).
• Cross-reactivity with mouse and rat ZHX2 has been observed; thus, it can be applied in rodent model systems. However, users should confirm species-specific reactivity in their experimental setup.
• As a polyclonal antibody, batch-to-batch variability may occur; it is recommended to titrate each new lot and include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, offering validated polyclonal antibodies like anti-ZHX2 to support research in transcriptional regulation and developmental processes. We supply bulk reagents, antibody pairs, and rare target raw materials, streamlining procurement from early-stage development to clinical applications.