Anti-ZGPAT Polyclonal Antibody for WB, ELISA - Q8N5A5

Core Product Specifications and Parameters

Biological Background: ZGPAT Function and Localization

• ZGPAT encodes two main isoforms: isoform 1 functions as a transcriptional repressor, while isoform 2 acts as a competitive antagonist of isoform 1-mediated repression by binding the NuRD complex without DNA-binding ability.
• Isoform 1 specifically binds the DNA consensus motif 5'-GGAG[GA]A[GA]A-3' and recruits the NuRD chromatin remodeling complex to target promoters, leading to transcriptional silencing.
• ZGPAT negatively regulates EGFR expression, a gene involved in cell proliferation, survival, and migration, suggesting a potential tumor suppressor role, particularly in breast carcinogenesis.
• The protein localizes predominantly to the nucleus, consistent with its function in transcriptional regulation.
• ZGPAT is widely expressed across tissues, indicating a broad regulatory role in gene expression.
• It features CCCH-type zinc finger and G patch domains, and is subject to post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 213–272 of human ZGPAT (NP_115916.3); this region is conserved in isoform 1 and may detect both endogenous and recombinant protein in Western blot.
• For Western blot, the predicted molecular weight is ~57 kDa. Consider using a blocking buffer such as 5% nonfat milk and titrating the primary antibody to reduce background.
• This antibody has shown reactivity with mouse ZGPAT; validation in the relevant sample system is recommended before use in other assays.
• In ELISA applications, the antibody can be tested as a coating or detection reagent; pairing with a suitable secondary antibody and optimizing the dilution are essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive sourcing platform for IVD raw materials, technical services, and consulting—from concept to clinic. For studies focused on transcriptional regulation and tumor biology, we supply high-quality antibodies like anti-ZGPAT, along with validated antibody pairs, optimized polyclonal and monoclonal reagents, and bulk ancillary materials. Our team can also assist in sourcing rare target raw materials to streamline your assay development pipeline.