Anti-ZFYVE9 Polyclonal Antibody for WB and ELISA - O95405

Core Product Specifications and Parameters

Biological Background: ZFYVE9 Function and Localization

• ZFYVE9 (Zinc finger FYVE domain-containing protein 9), also known as SARA (Smad anchor for receptor activation), is an early endosomal adaptor protein that belongs to the FYVE domain-containing family.
• It functions primarily in the TGF-β signaling pathway by recruiting SMAD2 and SMAD3 to the TGF-β receptor at the early endosome membrane, facilitating their phosphorylation and subsequent nuclear translocation.
• The protein modulates the transcriptional activity of the SMAD3/SMAD4 complex, thereby regulating downstream gene expression in response to TGF-β stimulation.
• ZFYVE9 may also be involved in TGF-β receptor internalization, contributing to signal attenuation and receptor trafficking.
• Subcellularly, ZFYVE9 is localized to the cytoplasm and specifically to the cytoplasmic side of the early endosome membrane, where its FYVE domain binds phosphatidylinositol 3-phosphate.
• Expression is ubiquitous, but in the brain it is predominantly found in cerebrovascular smooth muscle cells and reactive astrocytes, suggesting tissue-specific roles.
• The protein undergoes alternative splicing and is classified as a phosphoprotein, with a zinc-finger domain that mediates metal ion coordination, essential for its endosomal localization.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the N-terminal 260 amino acids; this region may be less conserved across splice variants, so validate detection of your target isoform in relevant cell lysates.
• For Western blotting, a band near 156 kDa is expected; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins, and include a positive control such as HeLa or HEK293 cell lysate.
• In ELISA, coat with recombinant ZFYVE9 protein or a peptide from the immunogen sequence; titrate the antibody to determine optimal working concentration and confirm specificity with a non-coated well.
• As a polyclonal antibody, it may recognize multiple epitopes; consider testing lot-to-lot consistency if used for quantitative assays, and always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, technical services, and consulting. For your TGF-β signaling and endosomal trafficking studies, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to support assay development from concept to clinic. Partner with us to streamline your procurement and accelerate your project timelines.