Anti-ZFPM2 Rabbit Polyclonal Antibody for WB, ELISA - Q8WW38

Core Product Specifications and Parameters

Biological Background: ZFPM2 Function and Localization

• Encodes zinc finger protein ZFPM2, also known as Friend of GATA protein 2 (FOG-2), which functions as a transcription regulator essential for heart morphogenesis and coronary vessel development from the epicardium.
• Acts as an essential cofactor by forming heterodimers with GATA family transcription factors GATA4, GATA5, and GATA6, modulating both activation and repression of target genes depending on cellular context.
• Plays a critical role in gonadal differentiation, potentially by regulating SRY gene expression, implicating it in sex determination pathways.
• Functions as a corepressor of NR2F2 (COUP-TFII), further expanding its role in transcriptional repression (By similarity).
• Localizes predominantly to the nucleus, consistent with its role as a transcription factor.
• Belongs to the zinc finger protein multitype family, containing multiple C2H2-type zinc fingers essential for DNA binding and protein-protein interactions.
• Widely expressed across tissues at low levels, as indicated by proteomics identification.
• Post-translational modifications include phosphorylation and ubiquitination (isopeptide bond), and alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 982-1151 within the C-terminal region of human ZFPM2; consider using this region for epitope mapping or competitive assays to verify specificity.
• For Western blot (WB), optimize lysate preparation from human or mouse tissues expressing ZFPM2 (e.g., heart, gonads) and anticipate a band around 128 kDa; include appropriate controls.
• In ELISA, titer optimization and cross-reactivity testing with other FOG family members (e.g., FOG1/ZFPM1) may be necessary for accurate detection.
• Validate antibody performance under native and denaturing conditions, as the target's conformation may influence epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive selection of IVD raw materials, including validated antibodies and ancillary reagents for transcription factor research. This anti-ZFPM2 rabbit polyclonal antibody supports studies in cardiac development and gonad differentiation, and can be integrated into larger diagnostic or research workflows. As a dedicated sourcing partner, CamelBio also provides access to rare-target raw materials, monoclonal antibody alternatives, and bulk reagent options to streamline your development from concept to clinical application.