Anti-ZFPM1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IX07

Core Product Specifications and Parameters

Biological Background: ZFPM1 Function and Localization

• ZFPM1 (Zinc finger protein multitype 1), also known as Friend of GATA protein 1 (FOG-1) or ZNF89A, is a multi‑zinc‑finger transcription cofactor critical for hematopoietic lineage commitment.
• It acts as an essential binding partner for GATA family transcription factors (GATA1, GATA2, GATA3), forming heterodimers that can either activate or repress target gene expression depending on cellular context.
• In the erythroid and megakaryocytic lineages, GATA1/FOG‑1 complexes drive activation of key genes such as NFE2, ITGA2B, and the alpha‑ and beta‑globin loci, while simultaneously repressing the erythroid regulator KLF1.
• ZFPM1 is predominantly localized in the nucleus, consistent with its function as a DNA‑tethering transcriptional regulator.
• Expression is mainly observed in hematopoietic tissues, with additional presence in adult cerebellum, stomach, lymph node, liver, pancreas, and fetal heart, liver, and spleen.
• The protein contains multiple C2H2‑type zinc‑finger domains that mediate protein‑protein interactions and DNA binding, and it is subject to phosphorylation, further modulating its activity.
• Beyond hematopoiesis, ZFPM1 is implicated in gonadal gene regulation and plays a non‑redundant role in cardiac development, distinct from its paralog ZFPM2/FOG2.

Experimental Guidance and Technical Tips

• The immunogen spans the N‑terminal 230 amino acids of human ZFPM1 isoform 1 (NP_722520.2), a region likely to be exposed in the native protein and suitable for generating reactivity against multiple isoforms.
• For western blotting, the predicted molecular weight is approximately 105 kDa; ensure adequate transfer conditions for high‑molecular‑weight proteins and validate band specificity using appropriate positive and negative controls.
• In immunofluorescence or immunocytochemistry, nuclear staining is expected; permeabilization steps should be optimized, and co‑staining with a GATA factor marker may help confirm localization.
• The antibody is polyclonal and recognizes human, mouse, and rat ZFPM1, but cross‑reactivity should be verified in the relevant sample system, as epitope conservation may vary across species.

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CamelBio provides diagnostic manufacturers and research laboratories with reliable access to validated IVD raw materials, including optimized polyclonal antibodies like this ZFPM1 rabbit pAb for hematopoiesis and transcription regulation studies. Our comprehensive portfolio covers validated antibody pairs, bulk ancillary reagents, and sourcing for rare‑target raw materials, supporting seamless progression from early‑stage research to clinical assay development. Partner with us to streamline your IVD supply chain with one‑stop convenience and technical expertise.