Anti-ZEB2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O60315

Core Product Specifications and Parameters

Biological Background: ZEB2 Function and Localization

• ZEB2 (Zinc finger E-box-binding homeobox 2), also known as SIP1, SMADIP1, or ZFHX1B, is a transcriptional repressor that binds to the E-box-like sequence 5'-CACCT-3' in different promoters. Related references: PMID:16061479, PMID:20516212
• It represses transcription of E-cadherin (CDH1), a key event in epithelial-mesenchymal transition (EMT). Related references: PMID:16061479
• ZEB2 also represses MEOX2, a homeobox transcription factor involved in limb muscle development. Related references: PMID:20516212
• The gene belongs to the ZEB family of two-handed zinc finger homeodomain transcription factors, which includes ZEB1 (TCF8).
• ZEB2 is localized to the nucleus and associates with chromatin, consistent with its role as a transcriptional regulator.
• The protein undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination, which may modulate its stability and activity.
• Mutations in ZEB2 are associated with Mowat-Wilson syndrome, characterized by Hirschsprung disease, intellectual disability, and epilepsy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 1050-1214) of human ZEB2, which includes multiple zinc finger motifs. Consider verifying antibody specificity using peptide blocking or knockout validation in your assay.
• For Western blotting, the predicted molecular weight is ~136 kDa. Use a low-percentage SDS-PAGE gel (e.g., 8%) and wet transfer with PVDF membrane for efficient detection of this large protein.
• In immunohistochemistry (paraffin), antigen retrieval using citrate buffer (pH 6.0) or EDTA buffer (pH 9.0) may be required. Optimize dilutions in the relevant tissue type; nuclear staining is expected.
• ELISA can be used for quantitative applications; follow standard sandwich or direct ELISA protocols with appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to IVD raw materials, including validated antibodies for transcription factors like ZEB2. From optimized polyclonal and monoclonal antibodies to bulk ancillary reagents, we streamline your supply chain for chromatin and EMT research. Rely on us for one-stop sourcing of rare targets and custom production needs.