Anti-ZEB1 Polyclonal Antibody for WB, IHC-P, ELISA - P37275

Core Product Specifications and Parameters

Biological Background: ZEB1 Function and Localization

• Zinc finger E-box-binding homeobox 1 (ZEB1), also known as TCF-8 or deltaEF1, is a transcriptional repressor that binds to E-box sequences (5'-CANNTG-3'). It plays a key role in regulating gene expression during development and disease.
• ZEB1 represses E-cadherin promoter activity and induces epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1, a process critical in cancer invasion and metastasis.
• It negatively regulates interleukin-2 (IL-2) gene expression and modulates ATP1A1 promoter activity in a context-dependent manner, acting as either a repressor or activator depending on cell type and cDNA levels.
• ZEB1 positively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis, highlighting its role in nervous system development.
• The protein contains zinc finger domains, a homeobox, and is subject to alternative splicing, phosphorylation, and ubiquitination, which may modulate its activity and stability.
• ZEB1 localizes to the nucleus and, at the protein level, is detected in E-cadherin-negative cells, stroma of normal colon, and at the invasion front of colorectal carcinomas. It is expressed in heart and skeletal muscle but not in liver, spleen, or pancreas.
• Keywords from UniProt include DNA-binding, metal-binding, phosphoprotein, and transcription regulation, reflecting its multifaceted regulatory functions.
• Mutations or altered expression of ZEB1 are associated with corneal dystrophies and various cancers, consistent with its roles in differentiation and EMT.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 490–710, a region encompassing zinc finger domains; consider this for epitope mapping or verification of specificity.
• For western blot, expect a band at approximately 124 kDa; validate detection in ZEB1-positive cell lines such as A549 or HCT116, and use appropriate controls.
• IHC-P may require optimization of antigen retrieval and detection conditions; tissues like colon carcinoma or heart, where ZEB1 expression is reported, can serve as positive controls.
• As a polyclonal antibody, lot-to-lot variability can occur; include appropriate normalization and controls in quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, offering access to validated antibody pairs, high-quality polyclonal and monoclonal antibodies, and bulk ancillary reagents. Our portfolio includes critical targets like ZEB1, supporting cancer research and diagnostic development. For rare or challenging targets, we specialize in custom sourcing to bridge gaps from concept to clinic.