Anti-ZEB1 Monoclonal Antibody for WB, ELISA - P37275

Core Product Specifications and Parameters

Biological Background: ZEB1 Function and Localization

• Zinc finger E-box-binding homeobox 1 (ZEB1), also known as TCF8, AREB6, and ZFHX1A, is a 124 kDa transcription factor belonging to the zinc finger homeodomain family.
• Functions primarily as a transcriptional repressor, inhibiting interleukin-2 (IL-2) gene expression, and repressing E-cadherin promoter to induce epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1.
• Regulates multiple developmental and pathological processes including neuronal differentiation and colorectal carcinoma progression, with tissue-specific expression in heart, skeletal muscle, and E-cadherin-negative cells.
• Localizes exclusively to the nucleus, consistent with its role in transcriptional regulation.
• Contains DNA-binding domains that recognize E-box motifs (5'-CANNTG-3'), enabling context-dependent repression or activation of target genes such as COL1A2 and ATP1A1.
• Post-translationally modified by phosphorylation and ubiquitination, as indicated by keywords Phosphoprotein and Ubl conjugation.
• Associated with corneal dystrophy and other disease variants when mutated, highlighting its clinical relevance.
• Alternative splicing generates isoforms with molecular weights of 116 kDa, 122 kDa, and 124 kDa, reflecting diverse regulatory functions.

Experimental Guidance and Technical Tips

• For Western blot detection, use nuclear extracts from human cell lines to enrich ZEB1, and anticipate multiple bands around 116–124 kDa due to isoform diversity.
• Pre-validate antibody reactivity in positive (e.g., ZEB1-expressing colorectal carcinoma) and negative control lysates to confirm specificity.
• For ELISA applications, optimize coating antigen and antibody dilution to achieve robust signal-to-noise ratios.
• Consider testing cross-reactivity with mouse or rat orthologs if required for experimental models, as the provided data specify human reactivity only.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated B2B supplier, CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials like this anti-ZEB1 monoclonal antibody. We support transcription factor and EMT research areas with validated antibody pairs, bulk ancillary reagents, and customized rare-target sourcing—helping you bridge the gap from discovery to application.