Anti-ZEB1 Monoclonal Antibody for WB, ELISA - P37275

Core Product Specifications and Parameters

Biological Background: ZEB1 Function and Localization

• ZEB1 is a zinc finger E-box-binding homeobox transcription factor that acts predominantly as a transcriptional repressor, regulating key developmental and disease processes.
• It plays a critical role in epithelial-mesenchymal transition (EMT) by repressing the E-cadherin promoter through recruitment of SMARCA4/BRG1, promoting invasive phenotypes.
• ZEB1 negatively regulates interleukin-2 (IL-2) gene expression and modulates the ATP1A1 promoter context-dependently, enhancing or repressing activity based on cellular conditions.
• In the absence of TGFB1, it represses COL1A2 transcription via E-box binding; it also represses BCL6 transcription in the presence of the corepressor CTBP1.
• ZEB1 positively regulates neuronal differentiation and represses RCOR1 transcription during neurogenesis, highlighting its role in neural development.
• It localizes strictly to the nucleus and exhibits tissue-specific expression: detected in heart and skeletal muscle, absent from liver, spleen, and pancreas. At the protein level, it colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells of colorectal carcinomas.
• The protein contains homeobox and zinc-finger domains, undergoes phosphorylation, ubiquitination, and alternative splicing, and is associated with diseases such as corneal dystrophy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 800–900 of human ZEB1; for Western blot, nuclear extracts are recommended given the target’s nuclear localization.
• For ELISA applications, the peptide-based design may be suitable for direct detection; confirm performance with recombinant ZEB1 or cell lysates from expressing lines.
• Cross-reactivity is reported for human and rat; validation in other species should be performed independently.
• Optimize antibody dilution and blocking conditions for your specific assay system; consider using a known nuclear protein loading control for WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research labs with seamless access to IVD raw materials, and this ZEB1 monoclonal antibody is a prime example for transcription factor and EMT studies. We supply validated antibody pairs, optimized monoclonals, ancillary reagents, and can source rare targets to support your entire workflow—from early research to clinical application. Let us bridge your concept to the clinic with reliable, bulk-scale antibody and reagent solutions.