Anti-ZDHHC5 Rabbit pAb for WB, ELISA - Q9C0B5

Core Product Specifications and Parameters

Biological Background: ZDHHC5 Function and Localization

• ZDHHC5 (Zinc finger DHHC domain-containing protein 5), also known as ZNF375, is a member of the DHHC palmitoyltransferase family.
• ZDHHC5 is a palmitoyltransferase that adds palmitate to substrates including CTNND2, CD36, GSDMD, NLRP3, NOD1, NOD2, STAT3, and S1PR1, regulating cell adhesion, inflammation, fatty acid uptake, bacterial sensing, and cardiac functions. Related references: PMID:21820437 PMID:29185452 PMID:31402609
• In synapses, ZDHHC5 palmitoylates delta-catenin/CTNND2 to enhance synaptic delivery and stabilization of AMPA receptors, with its membrane localization regulated by FYN phosphorylation and neuronal activity-dependent internalization. Related references: PMID:26334723
• ZDHHC5 contributes to innate immunity by palmitoylating NOD1 and NOD2, facilitating their recruitment to bacterial entry sites and phagosomes. Related references: PMID:31649195 PMID:34293401
• In fatty acid uptake, ZDHHC5 palmitoylates CD36, targeting it to the plasma membrane; subsequent fatty acid binding triggers LYN-mediated phosphorylation and inactivation of ZDHHC5, leading to CD36 endocytosis. Related references: PMID:32958780
• ZDHHC5 regulates inflammatory responses by palmitoylating NLRP3 to promote inflammasome assembly and activation, and by palmitoylating gasdermin-D (GSDMD) to promote its membrane translocation and pyroptotic pore formation. Related references: PMID:38092000 PMID:38530158 PMID:38599239
• ZDHHC5 controls oligodendrocyte development via STAT3 palmitoylation and may contribute to sperm tail assembly by palmitoylating IFT81 during spermatogenesis.
• ZDHHC5 localizes to the cell membrane, synapse, and Golgi apparatus. It is a multi-pass transmembrane protein subject to alternative splicing, methylation, and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 130-230 of human ZDHHC5, a region that may be conserved in mouse; the stated cross-reactivity with mouse should be validated in appropriate sample systems.
• For Western blotting, consider the predicted molecular weight of approximately 78 kDa. Validate band specificity using appropriate controls and, if necessary, peptide blocking experiments.
• For ELISA applications, the immunizing peptide can serve as a useful positive control to assess antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering a comprehensive portfolio of validated antibodies, recombinant proteins, and ancillary reagents. This ZDHHC5 polyclonal antibody supports research in neuroscience, immunology, and metabolic disorders, and can be procured along with custom bulk antibody production and technical support to streamline your assay development from concept to clinic.