Anti-ZDHHC4 Rabbit Polyclonal Antibody for WB and ELISA - Q9NPG8

Core Product Specifications and Parameters

Biological Background: ZDHHC4 Function and Localization

• Palmitoyltransferase that catalyzes protein palmitoylation, a reversible lipid modification regulating protein localization and function.
• Substrates include the D(2) dopamine receptor DRD2, GSK3B, and MAVS, linking ZDHHC4 to neurotransmission and immune signaling.
• Mediates GSK3B palmitoylation at the endoplasmic reticulum, preventing AKT1-mediated phosphorylation and promoting STAT3 signaling pathway activation. Related references: PMID:35606353
• Catalyzes MAVS palmitoylation, which stabilizes MAVS and promotes antiviral signaling by altering its ubiquitination pattern (inhibiting Lys-48-linked and facilitating Lys-63-linked ubiquitination). Related references: PMID:38016475
• Localizes to the endoplasmic reticulum membrane, Golgi apparatus membrane, and cell membrane, consistent with its role in secretory pathway protein modifications.
• Belongs to the DHHC family of palmitoyltransferases, characterized by a zinc finger DHHC domain essential for enzymatic activity.
• Implicated in various cellular processes including protein trafficking, signal transduction, and membrane organization.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region between amino acids 110-210 of human ZDHHC4; consider using this peptide for competition assays to confirm antibody specificity.
• For Western blot, a band of approximately 40 kDa is expected; we recommend validating with lysates from human, mouse, or rat cell lines and using appropriate blocking and detection conditions.
• In ELISA, ensure optimal coating of the antigen and titrate the antibody to determine the best working concentration.
• Due to high sequence conservation, cross-reactivity with mouse and rat ZDHHC4 is predicted; validate in these species using your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive supplier of IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents to support diagnostic development from concept to clinic. For palmitoylation and signal transduction research, we provide high-quality reagents like this anti-ZDHHC4 antibody, along with rare target sourcing capabilities. Partner with CamelBio to streamline your assay development and ensure reliable, scalable raw material supply.