Anti-ZDHHC20 Rabbit Polyclonal Antibody for WB and ELISA - Q5W0Z9

Core Product Specifications and Parameters

Biological Background: ZDHHC20 Function and Localization

• ZDHHC20 (also known as DHHC20) is a palmitoyltransferase belonging to the DHHC domain-containing cysteine-rich protein family, characterized by a zinc finger motif and multiple transmembrane helices.
• It catalyzes the addition of palmitate onto various protein substrates, including EGFR, TRIM28, and CALHM1, playing roles in signal transduction, DNA repair, and ion channel modulation (PubMed:27153536, PubMed:29326245, PubMed:33219126, PubMed:41109928). Related references: PMID:27153536 PMID:29326245 PMID:33219126 PMID:41109928
• Specifically palmitoylates cysteine residues in the cytoplasmic C-terminus of EGFR, thereby modulating EGFR internalization, degradation, and the duration of downstream signaling (PubMed:27153536). Related references: PMID:27153536
• Displays substrate preference for C16 acyl-CoA but can also utilize C14 and C18 fatty acid chains (PubMed:29326245). Related references: PMID:29326245
• Plays a critical role in the DNA damage repair response by palmitoylating TRIM28 (PubMed:41109928). Related references: PMID:41109928
• During SARS-CoV-2 infection, ZDHHC20 is the dominant palmitoyltransferase responsible for spike protein lipidation, promoting spike biogenesis, stability, and viral fusion.
• Localizes to the Golgi apparatus, cell membrane, endoplasmic reticulum, and perinuclear region, consistent with its roles in protein trafficking and membrane organization.
• May palmitoylate CALHM1 subunit of gustatory voltage-gated ion channels, influencing channel gating and kinetics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270-365 of human ZDHHC20; consider that sequence homology across species supports reactivity with mouse and rat orthologs.
• For Western blot, validate the antibody with positive control lysates from cell lines expressing ZDHHC20 (e.g., HeLa, A549) and use appropriate loading controls.
• In ELISA applications, peptide competition assays can help confirm binding specificity.
• As a polyclonal antibody, lot-to-lot consistency should be verified; consider including a reference standard in each experiment.
• Since ZDHHC20 is a multi-pass membrane protein, gentle lysis conditions and proper sample preparation (e.g., avoiding boiling) may preserve epitope accessibility.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, supporting your development from concept to clinic. In the field of palmitoyltransferases and host-virus interaction research, we provide validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. Our expertise ensures consistent supply and technical support for demanding assay formats.