Anti-ZDHHC2 Rabbit Polyclonal Antibody for WB and ELISA - Q9UIJ5

Core Product Specifications and Parameters

Biological Background: ZDHHC2 Function and Localization

• ZDHHC2, also known as DHHC2, ZNF372, REC and REAM, is a member of the DHHC family of palmitoyltransferases that catalyze the attachment of palmitate to cysteine residues on target proteins.
• It exhibits broad fatty acid selectivity, transferring not only palmitate but also myristate and stearate onto substrates, thereby regulating protein trafficking, membrane localization and function.
  Related references: PMID:18296695 / PMID:18508921 / PMID:19144824
• In the nervous system, ZDHHC2 palmitoylates AKAP5, DLG4 and RGS7BP, playing a key role in synaptic plasticity by controlling AMPA receptor clustering and GPCR signaling in dendrites.
• ZDHHC2 regulates cell adhesion by palmitoylating CD9 and CD151, thereby controlling their surface expression and function.
  Related references: PMID:18508921
• It palmitoylates the endoplasmic reticulum protein CKAP4, promoting its translocation to the plasma membrane, and may also modify LCK to influence immune signaling.
  Related references: PMID:18296695 / PMID:19144824 / PMID:22034844
• Subcellularly, ZDHHC2 localizes to the postsynaptic density, recycling endosomes, cell membrane, ER and Golgi membranes, consistent with its roles in protein trafficking and signaling.
• ZDHHC2 is ubiquitously expressed, with notably reduced levels observed in colorectal cancers with liver metastasis, linking it to tumor progression.
  Related references: PMID:10918388 / PMID:22034844

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 270–367 of human ZDHHC2. The resulting antiserum is expected to recognize full-length ZDHHC2 and isoforms containing this region; however, cross-reactivity with other family members should be assessed.
• For Western blotting, a band of approximately 42 kDa is anticipated. Include appropriate controls (e.g., ZDHHC2‑overexpressing lysates) and optimize sample preparation for this multi‑pass transmembrane protein.
• In ELISA, the antibody can serve as a capture or detection reagent. Pair with a compatible secondary antibody and validate with recombinant protein or known positive samples.
• Since ZDHHC2 is palmitoylated and membrane‑associated, consider using detergent‑containing lysis buffers and avoid boiling if epitope sensitivity is a concern.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we empower diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials for the entire workflow—from concept to clinic. For your palmitoylation and cancer metastasis research, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-ZDHHC2 rabbit pAb, bulk ancillary reagents, and specialized sourcing for rare targets. Partner with us to accelerate your assay development with reliable, ready‑to‑scale solutions.