Anti-ZDHHC18 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NUE0

Core Product Specifications and Parameters

Biological Background: ZDHHC18 Function and Localization

• Palmitoyltransferase that catalyzes the addition of palmitate onto various protein substrates, including CGAS, HRAS, and LCK. Related references: PMID:23034182 PMID:27481942 PMID:35438208
• Acts as a negative regulator of the cGAS-STING pathway by mediating palmitoylation and inactivation of CGAS, linking it to innate immunity. Related references: PMID:35438208
• May also have palmitoyltransferase activity toward the beta-2 adrenergic receptor (ADRB2), potentially regulating G protein-coupled receptor signaling. Related references: PMID:27481942
• Localizes to the Golgi apparatus membrane.
• Widely expressed across tissues.
• Belongs to the DHHC family of palmitoyltransferases; contains a DHHC domain and multiple transmembrane helices.
• Keywords highlight roles in acyltransferase activity, immunity, and membrane association.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region near the C-terminus (amino acids 301-388); this region may be conserved across isoforms, aiding in pan-isoform detection.
• For Western blot, consider a standard dilution range (e.g., 1:500-1:2000) and validate with appropriate positive and negative controls in your sample system. The predicted molecular weight is ~42 kDa; additional bands may appear due to palmitoylation or post-translational modifications.
• IHC-P may require antigen retrieval and optimization; test on known positive tissues (e.g., tumors or immune cell-rich samples) given wide expression.
• ELISA performance depends on coating buffer and detection conditions; consider pairing with a suitable detection antibody for sandwich ELISA.
• When working with mouse or rat samples, confirm reactivity under your experimental conditions, as cross-reactivity is supplier-claimed but should be independently verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized IVD raw material supplier, empowering diagnostic manufacturers and researchers with reliable access to validated antibodies, bulk reagents, and rare target sourcing. This ZDHHC18 antibody supports investigations into palmitoylation-mediated signaling and innate immunity. From concept to clinic, CamelBio streamlines your procurement pipeline, ensuring timely delivery of quality raw materials to accelerate your assay development.