Anti-ZDHHC15 Rabbit Polyclonal Antibody for WB, ELISA - Q96MV8

Core Product Specifications and Parameters

Biological Background: Palmitoyltransferase ZDHHC15 Function and Localization

• ZDHHC15 is a member of the zinc finger DHHC domain-containing protein family, functioning as an acyltransferase that catalyzes protein palmitoylation.
• It mediates the addition of palmitate onto various protein substrates and can also transfer myristate and stearate, displaying broad fatty acid selectivity.
• Palmitoylates IGF2R and SORT1, facilitating their partitioning to endosomal membrane subdomains where they interact with the retromer cargo-selective complex, thereby regulating retrograde transport of lysosomal sorting receptors from endosomes to the Golgi. Related references: PMID:18817523
• In the nervous system, it palmitoylates DLG4/PSD95 to regulate synaptic clustering and synaptogenesis, and may also palmitoylate GAP43, DNAJC5, CALHM3, and FYN (PubMed:19956733); involved in dopaminergic neuron differentiation and diencephalon development. Related references: PMID:19956733
• Localizes predominantly to the Golgi apparatus membrane and postsynaptic density.
• Widely expressed in placenta, liver, lung, kidney, heart, and brain; alternative splicing generates multiple isoforms (predicted weights 17 kDa, 38 kDa, 39 kDa), and mutations are associated with X-linked intellectual disability (MRX91).

Experimental Guidance and Technical Tips

• For Western blotting, consider that multiple isoforms may be detected (17 kDa, 38 kDa, 39 kDa); validate the observed band pattern in your specific sample system.
• Given the peptide immunogen (amino acids 231-331), epitope availability may vary across isoforms; pre-adsorption controls are advisable to confirm specificity.
• In ELISA, the synthetic immunogen peptide can serve as a positive control; optimize coating concentrations and validate with known positive and negative samples.
• Since this is a polyclonal antibody, evaluate lot-to-lot consistency for critical applications.
• Cross-reactivity is reported for human; for other species, check sequence homology of the immunogen region if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your procurement of IVD raw materials by offering this anti-ZDHHC15 antibody alongside validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary supplies. With expertise in protein palmitoylation and intracellular trafficking targets, we help diagnostic manufacturers and labs secure rare-target raw materials and accelerate assay development. Reach out to explore how CamelBio can be your sourcing partner from concept to clinic.