Anti-ZCWPW1 Polyclonal Antibody for WB, ELISA - Q9H0M4

Core Product Specifications and Parameters

Biological Background: ZCWPW1 Function and Localization

• ZCWPW1 (Zinc finger CW-type PWWP domain protein 1) is a dual histone methylation reader that specifically recognizes PRDM9-catalyzed histone marks H3K4me3 and H3K36me3 (PubMed:20826339, PubMed:32744506). Related references: PMID:20826339, PMID:32744506
• It facilitates the repair of PRDM9-induced meiotic double-strand breaks (DSBs), which is crucial for proper meiotic recombination (By similarity).
• Essential for male fertility and spermatogenesis, and required for meiosis prophase I progression specifically in male germ cells (By similarity).
• The protein localizes to the nucleus and chromosomes, consistent with its role in chromatin-associated processes during meiosis.
• Contains zinc-finger domains and a PWWP domain, indicating its function in metal-binding and protein-DNA interactions, and is subject to phosphorylation (based on keywords).
• Predominantly expressed in testis, reflecting its specialized role in spermatogenesis.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 389-648 of human ZCWPW1, which covers domains important for histone reading; this design may enhance specificity for the target protein.
• For Western blotting (WB), expect a band at approximately 72 kDa in human and mouse testis lysates or relevant cell lines. Validate with appropriate positive and negative controls.
• Due to the testis-specific expression, consider using testis tissue as a positive control for WB; cross-reactivity with mouse allows for comparative studies.
• For ELISA applications, consider optimizing antigen coating conditions and antibody dilution to achieve optimal signal-to-noise ratios.
• Although not tested in other applications, this polyclonal antibody may be explored for immunoprecipitation or immunofluorescence based on target localization in the nucleus.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing platform for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-ZCWPW1 antibody, and ancillary reagents. Our supply capabilities support research in meiotic recombination, spermatogenesis, and epigenetics, ensuring seamless progression from concept to clinic. We also offer technical consulting and rare target material sourcing to accelerate your assay development.