Anti-ZCCHC7 Rabbit Polyclonal Antibody for WB, ELISA - Q8N3Z6

Core Product Specifications and Parameters

Biological Background: ZCCHC7 Function and Localization

• ZCCHC7 (Zinc finger CCHC domain-containing protein 7), also designated TRAMP-like complex RNA-binding factor ZCCHC7, is a zinc-finger protein encoded by the ZCCHC7 gene on human chromosome 2.
• The protein localizes predominantly to the nucleolus within the nucleus, as annotated in UniProt, consistent with roles in RNA processing and ribosome biogenesis.
• ZCCHC7 comprises multiple CCHC-type zinc finger repeats, which likely mediate RNA or DNA binding through coordination of zinc ions.
• Post-translational modifications include phosphorylation and ubiquitin-like (Ubl) conjugation (isopeptide bonds), which may modulate its activity or stability.
• Alternative splicing of ZCCHC7 mRNA generates multiple isoforms, potentially contributing to functional diversity.
• The protein has been identified in mass spectrometry-based proteomics studies, confirming its expression in human cells.
• Human ZCCHC7 has a calculated molecular weight of approximately 63 kDa (UniProt: 63,052 Da), consistent with the detected band size.
• Its mouse and rat orthologs share high sequence similarity, supporting the observed cross-reactivity of this antibody.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal amino acids 1–231, which may enable detection of multiple ZCCHC7 isoforms; consider using a blocking peptide or recombinant protein to confirm specificity.
• This polyclonal antibody is suitable for western blot (WB) and ELISA; optimal dilutions should be determined empirically in the target sample system.
• Cross-reactivity with mouse and rat is indicated; validate performance in the relevant species’ tissue or cell lysates, as endogenous expression levels may vary.
• For subcellular localization experiments, note the nucleolar enrichment of ZCCHC7; include appropriate nuclear and nucleolar markers as controls.
• Recombinant immunogen and rabbit host ensure consistent batch-to-batch reproducibility for IVD assay development.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio that includes validated polyclonal antibodies like this ZCCHC7 pAb, optimized monoclonal antibodies, antibody pairs, and bulk ancillary reagents.
• Our expertise extends to rare and hard-to-find target materials, supporting diagnostic manufacturers and research laboratories from early-stage assay development through clinical validation.
• Whether you need consistent supply of this ZCCHC7 antibody for nucleolar RNA processing research or custom material solutions, CamelBio streamlines procurement to accelerate your diagnostic workflow.