Anti-ZCCHC3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NUD5

Core Product Specifications and Parameters

Biological Background: ZCCHC3 Function and Localization

• ZCCHC3 (Zinc finger CCHC domain-containing protein 3, also known as C20orf99) is a nucleic acid-binding protein that plays an important role in the innate immune response to both DNA and RNA viruses. Related references: PMID:30135424 PMID:30193849

• Functions as a co-sensor for cytosolic double-stranded DNA (dsDNA) by cGAS, enhancing cGAS oligomerization and activation to promote antiviral signaling. Related references: PMID:30135424

• Promotes recognition of viral RNA by RIGI and IFIH1/MDA5 through direct binding to double-stranded RNA (dsRNA) and facilitating 'Lys-63'-linked ubiquitination of these sensors. Related references: PMID:30193849

• Contains CCHC-type zinc finger domains that mediate nucleic acid binding and metal ion coordination.

• Localizes predominantly in the cytoplasm where it interacts with viral nucleic acids and pattern recognition receptors.

• Annotated with keywords including Alternative splicing, Antiviral defense, DNA-binding, Immunity, Innate immunity, Phosphoprotein, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a recombinant fragment corresponding to amino acids 215-335 of human ZCCHC3. As a polyclonal antibody, it may recognize multiple epitopes within this region.
• For Western blotting, the predicted molecular weight of ZCCHC3 is approximately 44 kDa. Ensure proper loading controls and consider the possibility of post-translational modifications that may alter migration.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic staining consistent with the reported subcellular localization of ZCCHC3. Include appropriate controls such as secondary antibody alone and knockout/knockdown validation if available.
• This antibody is also suitable for ELISA, which can be used for quantitative detection of the immunogen or recombinant ZCCHC3. Confirm binding specificity with known positive and negative controls.
• Since cross-reactivity with mouse is documented, it may be applicable for mouse model studies; however, verify performance in your specific tissue or cell line to ensure consistent results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers by providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and targeted sourcing assistance for rare targets. For researchers investigating innate immunity pathways, our ZCCHC3 antibody offers a reliable tool to study nucleic acid sensing mechanisms and antiviral responses. Contact us to explore scalable supply options and complementary reagents tailored to your assay development needs.