Anti-ZCCHC11 Polyclonal Antibody for WB, IF/ICC, ELISA - Q5TAX3

Core Product Specifications and Parameters

Biological Background: ZCCHC11 Function and Localization

• Terminal uridylyltransferase 4 (TUT4), also known as zinc finger CCHC domain-containing protein 11 (ZCCHC11), PAPD3 or TENT3A, is a nucleotidyltransferase enzyme responsible for adding uridine residues to the 3' ends of RNA substrates.
• Mediates terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, thereby promoting global mRNA decay. Related references: PMID:25480299 PMID:31036859
• Plays a dual role in microRNA (miRNA) biology: suppresses miRNA biogenesis by uridylating precursor miRNAs (e.g., pre-let-7) leading to degradation, and also facilitates miRNA-induced gene silencing by uridylating deadenylated miRNA targets; in absence of LIN28A, monouridylates group II pre-miRNAs to enhance Dicer processing. Related references: PMID:25979828 PMID:19703396
• Essential for oocyte maturation and fertility; collaborates with TUT7 to sculpt the maternal transcriptome via mRNA uridylation during oocyte growth.
• Functions in cytoplasmic ribonucleoprotein granules to restrict LINE-1 retrotransposition by destabilizing LINE-1 mRNAs, cooperating with MOV10. Related references: PMID:30122351
• Suppresses Toll-like receptor-induced NF-kappa-B activation through interaction with T2BP. Related references: PMID:16643855
• Localizes to the nucleus, cytoplasm, and cytoplasmic ribonucleoprotein granules, consistent with its roles in RNA metabolism and stress response.
• The protein contains CCHC zinc finger domains and requires divalent metal ions (Mg2+, Mn2+) for catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-290) of human ZCCHC11; reactivity is confirmed against human, mouse, and rat. Validate species-specific expression in your experimental model.
• For western blotting, the predicted molecular weight is ~185 kDa; use a low-percentage SDS-PAGE gel (e.g., 6-8%) and optimize transfer conditions for high-molecular-weight proteins.
• In IF/ICC, anticipate both nuclear and cytoplasmic signals; consider appropriate fixation and permeabilization protocols to preserve antigenicity.
• Antibody performance should be verified in target applications (WB, IF/ICC, ELISA) under laboratory-specific conditions; titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated polyclonal antibodies such as this anti-ZCCHC11 antibody for mRNA uridylation and miRNA biogenesis investigations. Our one-stop platform also offers matched antibody pairs, engineered monoclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—helping accelerate your assay development and clinical translation projects.