Anti-ZC3H7A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q8IWR0

Core Product Specifications and Parameters

Biological Background: Zinc finger CCCH domain-containing protein 7A Function and Localization

• Zinc finger CCCH domain-containing protein 7A (ZC3H7A), also known as ZC3H7 or ZC3HDC7, is encoded by the ZC3H7A gene in humans.
• ZC3H7A is a putative regulator of microRNA (miRNA) biogenesis. It binds to the hairpin structures of MIR7-1, MIR16-2, and MIR29A, specifically recognizing the 3'-ATA(A/T)-5' motif in the apical loop.
• The protein localizes to the nucleus, consistent with its role in miRNA processing.
• ZC3H7A contains CCCH-type zinc finger domains, TPR repeats, and coiled coil regions, and is capable of RNA binding and metal binding via its zinc fingers.
• It undergoes alternative splicing and is identified as a phosphoprotein in proteomics studies, suggesting post-translational regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-220) of human ZC3H7A. For Western blot, a band around 111 kDa is predicted; validate with appropriate positive and negative lysates.
• For immunofluorescence/ICC, use permeabilization to allow nuclear access. Include a nuclear counterstain and consider a blocking step to reduce background.
• In ELISA applications, the antibody may be used for detection; optimize coating antigen (recombinant protein or peptide) and antibody dilutions.
• Cross-reactivity with mouse and rat is reported, but confirm sequence homology and validate in each species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines the procurement of premium IVD raw materials, offering a comprehensive portfolio including validated antibody pairs, bulk monoclonal and polyclonal antibodies, and ancillary reagents. For targets such as ZC3H7A involved in miRNA biogenesis and nuclear processes, CamelBio provides reliable sourcing of specific research reagents to support diagnostic and R&D workflows from concept to clinic.