Anti-ZC3H15 Polyclonal Antibody for WB, ELISA - Q8WU90

Core Product Specifications and Parameters

Biological Background: ZC3H15 Function and Localization

• ZC3H15 (Zinc finger CCCH domain-containing protein 15) is also known as DFRP1 (DRG family-regulatory protein 1) and LEREPO4 (likely ortholog of mouse immediate early response erythropoietin 4) (UniProt Q8WU90).
• It functions as a protective factor for DRG1 (developmentally regulated GTP-binding protein 1), preventing its proteolytic degradation. Related references: PMID:19819225
• ZC3H15 stimulates DRG1 GTPase activity, likely by enhancing the affinity for potassium ions. Related references: PMID:23711155
• The protein localizes to both the cytoplasm and nucleus (UniProt Q8WU90).
• ZC3H15 contains CCCH-type zinc finger domains and coiled-coil regions, characteristic of RNA-binding regulatory proteins (UniProt Q8WU90).
• Post-translational modifications include phosphorylation and methylation, suggesting dynamic regulation (Keywords: Phosphoprotein, Methylation).
• The protein undergoes alternative splicing, potentially giving rise to multiple isoforms with distinct functional properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-300) of human ZC3H15; thus, the antibody may recognize full-length and N-terminal fragments across species.
• For Western blot (WB), the predicted molecular weight is approximately 49 kDa, but alternative splicing may produce additional bands; validate in your target sample system.
• In ELISA applications, consider using recombinant ZC3H15 or peptide fragments as coating antigens to assess specificity.
• When working with mouse samples, cross-reactivity is reported; however, perform a species-specific control to confirm reactivity patterns.
• Optimize antibody dilution and incubation conditions based on your specific assay and sample type, as no standardized protocol is provided.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, supplying validated antibodies like this ZC3H15 polyclonal antibody alongside a comprehensive portfolio for diagnostic development. Whether you need optimized antibody pairs, bulk ancillary reagents, or rare target sourcing for DRG1/GTPase research, CamelBio supports your workflow from concept to clinic. Contact us to streamline your procurement and accelerate your diagnostic projects.