Anti-ZC3H14 Polyclonal Antibody for WB and ELISA - Q6PJT7

Core Product Specifications and Parameters

Biological Background: ZC3H14 Function and Localization

• ZC3H14 is an RNA-binding protein involved in the biogenesis of circular RNAs (circRNAs) by binding exon-intron boundaries and 3'-UTRs of pre-mRNAs. Related references: PMID:39461343
• It promotes circRNA formation through dimerization and association with the spliceosome, a process essential for spermatogenesis. Related references: PMID:39461343
• ZC3H14 regulates pre-mRNA processing of ATP5MC1, preventing its degradation. Related references: PMID:27563065
• The protein binds the poly(A) tail of mRNAs, controlling poly(A) length in neuronal cells. Related references: PMID:17630287 PMID:24671764
• Localizes to nuclear speckles and can also be found in the cytoplasm.
• Predominantly expressed in the brain, including fetal and adult temporal lobe.
• Contains zinc finger CCCH domains and undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitination.
• Mutations in ZC3H14 are linked to intellectual disability (MRT56).

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal region (amino acids 1-306), potentially recognizing full-length ZC3H14 and N-terminal fragments; consider this when interpreting banding patterns.
• For Western blotting, the predicted molecular weight is approximately 83kDa; however, validate the observed band size in your experimental system and consider post-translational modifications.
• Cross-reactivity with mouse and rat ZC3H14 is supported; optimize antibody dilutions for each species and tissue type.
• This polyclonal antibody is suitable for ELISA; include appropriate controls and check lot-to-lot consistency for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For ZC3H14 research in circRNA biology, spermatogenesis, and neurobiology, we offer reliable anti-ZC3H14 antibodies and can assist in sourcing rare-target raw materials to support your assay development and production needs.