Anti-ZC3H12D Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - A2A288

Core Product Specifications and Parameters

Biological Background: ZC3H12D Function and Localization

• ZC3H12D (also known as MCPIP4, p34, TFL) is a probable ribonuclease featuring a CCCH-type zinc finger domain, and it belongs to the ZC3H12 family of RNases.
• It may regulate cell growth by suppressing RB1 phosphorylation, thereby inhibiting G1 to S phase progression; this activity suggests a tumor suppressor role. Related references: PMID:19531561
• It serves as a tumor suppressor in certain leukemia cells. Related references: PMID:17210687
• Together with ZC3H12A, it enhances the degradation of interleukin IL-6 mRNA in activated macrophages, indicating a role in inflammatory response regulation. Related references: PMID:26134560
• ZC3H12D localizes predominantly to the cytoplasm and P-bodies, and also shuttles to the nucleus, consistent with its role in mRNA decay.
• Expression is found in normal lymphocytes but is defective in some leukemia/lymphoma cell lines, implicating it in lymphocyte biology and hematopoietic malignancies.
• It binds metal ions, likely magnesium, which is characteristic of its nuclease activity.
• Alternative splicing and chromosomal rearrangement events are associated with this gene, possibly contributing to its dysregulation in cancer.

Experimental Guidance and Technical Tips

• For WB, this polyclonal antibody may detect endogenous ZC3H12D at ~58 kDa. Consider using whole-cell extracts from lymphocyte or leukemia cell lines as positive controls.
• For IF/ICC, expect cytoplasmic and nuclear staining; P-body localization may be observed under specific conditions. Validate fixation/permeabilization methods for optimal results.
• For ELISA, the antibody can be paired with appropriate detection reagents; consider titration for best signal-to-noise ratio.
• Since the immunogen corresponds to a recombinant fusion protein within the central region (aa150-230), the antibody may recognize multiple isoforms containing this epitope; confirm specificity by siRNA knockdown or using knockout models if available.
• Cross-reactivity with mouse and rat is reported; validate in the species-specific experimental context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for IVD raw-material sourcing, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers studying tumor suppression or inflammatory pathways, we provide reliable access to ZC3H12D antibodies and related antigens. Our one-stop platform bridges your concept-to-clinic needs with technical consulting and efficient procurement support.