Anti-ZC3H12C Rabbit Polyclonal Antibody (MCPIP3) for WB, IF/ICC, ELISA - Q9C0D7

Core Product Specifications and Parameters

Biological Background: ZC3H12C Function and Localization

• ZC3H12C, also known as MCPIP3 (MCP-induced protein 3), is a zinc finger CCCH domain-containing protein encoded by the ZC3H12C gene.
• It is classified as a probable ribonuclease and may function in regulating the levels of target RNA species.
• The protein contains a CCCH-type zinc-finger domain, characteristic of RNA-binding proteins with endonuclease activity.
• Keywords from UniProt include endonuclease, hydrolase, nuclease, magnesium, and metal-binding, indicating its catalytic mechanism likely depends on divalent cations.
• Alternative splicing generates multiple isoforms of ZC3H12C, and it is identified as a phosphoprotein through proteomic analyses.
• The molecular weight of the canonical isoform is approximately 99 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 735–883 near the C-terminus; the antibody may recognize full-length ZC3H12C and some splice variants.
• For Western blot, the predicted band size is ~99 kDa; consider using a 10% SDS-PAGE gel and appropriate transfer conditions.
• Include a positive control such as human cell lines known to express ZC3H12C (e.g., HeLa, HEK293) to validate detection.
• For immunofluorescence/immunocytochemistry, optimize fixation and permeabilization protocols for your specific cell type.
• When testing cross-reactivity with mouse samples, confirm reactivity using a relevant tissue or cell lysate, as epitope conservation may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, offering one-stop access to IVD raw materials and technical support. This anti-ZC3H12C polyclonal antibody supports studies in RNA biology and gene regulation. Alongside validated monoclonal and polyclonal antibodies, CamelBio supplies bulk ancillary reagents and assists with sourcing rare targets, streamlining your path from concept to clinic.