Anti-ZBTB8A Rabbit Polyclonal Antibody for WB, ELISA - Q96BR9

Core Product Specifications and Parameters

Biological Background: ZBTB8A Function and Localization

• ZBTB8A, also known as BOZF1, is a zinc finger and BTB domain-containing protein that belongs to the ZBTB transcription factor family.
• The protein contains an N-terminal BTB/POZ domain typically involved in protein-protein interactions and transcriptional repression, as well as C-terminal C2H2-type zinc finger domains likely mediating DNA binding.
• UniProt functional annotation suggests that ZBTB8A may be involved in transcriptional regulation.
• The protein is predicted to localize to the nucleus, consistent with a role in gene expression control.
• Alternative splicing generates multiple isoforms; the antibody targets a region common to the canonical isoform.
• Post-translational modifications include phosphorylation and ubiquitination, which may modulate its activity (Keywords: Phosphoprotein, Ubl conjugation).
• No tissue-specific expression data is available, but its role as a transcription factor implies broad regulatory potential.
• Keywords: Alternative splicing, DNA-binding, Isopeptide bond, Metal-binding, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Repeat, Transcription, Transcription regulation, Ubl conjugation, Zinc, Zinc-finger.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 200-400 of human ZBTB8A, covering the region between the BTB/POZ domain and zinc finger repeats. For WB, the antibody is expected to detect a band near 50 kDa; run appropriate positive controls (e.g., human, mouse, rat cell lysates) and optimise blocking/dilution conditions.
• For ELISA, indirect or sandwich formats using recombinant ZBTB8A protein or peptides can be employed to validate reactivity and specificity. Pair with species-matched secondary detection reagents.
• Since cross-reactivity is reported for human, mouse, and rat, verify species-specific binding in your experimental system and include proper loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with streamlined access to high-quality IVD raw materials like anti-ZBTB8A antibodies. Beyond this transcription factor target, we supply validated antibody pairs, optimised monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare raw materials for zinc-finger protein research. Let our one-stop capability accelerate your transition from concept to clinic.